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Skull strip the T1 image
Michelle Voss edited this page Oct 26, 2023
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By the end of this practical you should be able to complete preprocessing on your data with the following steps:
- use FSL's bet tool to remove the brain from a T1 image
- use FSLeyes to check the output of
bet
Access FastX through the remote login:
https://fastx.divms.uiowa.edu:3443/
In the terminal move to the folder where our downloaded data is:
- A the terminal prompt type:
cd fmriLab - Type
lsand you should see the data you downloaded as a folder namedflankerData_n4
Using FSL's brain extraction tool to skull-strip:
- At the prompt type
fsl - Click the "BET brain extraction" button on the FSL menu
- Steps to set up
BET- Click the yellow folder to select your defaced T1 image with skull as your
Input Image - FSL will automatically fill in the
Output Imageto have the same file name as your T1 with_brainat the end - Expand
Advanced Options- Select
Output binary brain mask image
- Select
- Select
Goto runBET
- Click the yellow folder to select your defaced T1 image with skull as your
- Example default settings
**Checking BET:**
* Use the GUI menu to open your defaced T1 and brain mask in FSLeyes * Use the `Opacity` slider to make the mask transparent so you can see the brain image in the background * Checklist * [ ] Top of the brain: does the mask cover the brain and not spill into the eyes or skull? * [ ] Bottom of the brain: does the mask "hug" the brainstem or spill into the throat?
**Modifying BET settings:**
* Open or go back to the BET GUI * An advanced option that always helps: Use the dropdown menu to select `Robust brain centre estimation` * If your mask was too small or large at the top of the brain, change the `Fractional intensity threshold` * If your mask was too small or large at either the top or the bottom of the brain, change the `Threshold gradient` 