@@ -142,50 +142,50 @@ RUN wget https://github.com/alexdobin/STAR/releases/download/2.7.11b/STAR_2.7.11
142142 cp STAR_2.7.11b/Linux_x86_64/STAR* /usr/local/bin/ && \
143143 rm -rf STAR_2.7.11b*
144144
145- # Install QualiMap (resilient)
146- RUN set -eux; \
147- curl -fSL --retry 5 --retry-connrefused --retry-delay 3 \
148- -o qualimap_v2.3.zip \
149- https://bitbucket.org/kokonech/qualimap/downloads/qualimap_v2.3.zip \
150- || curl -fSL --retry 5 --retry-connrefused --retry-delay 3 \
151- -o qualimap_v2.3.zip \
152- http://qualimap.conesalab.org/qualimap_v2.3.zip; \
153- unzip -q qualimap_v2.3.zip; \
154- mv qualimap_v2.3 /opt/qualimap; \
155- printf '%s\n ' '#!/bin/bash' 'exec java -Xms32m -Xmx2g -jar /opt/qualimap/qualimap.jar "$@"' > /usr/local/bin/qualimap; \
156- chmod +x /usr/local/bin/qualimap; \
157- rm -f qualimap_v2.3.zip
145+ # # Install QualiMap (resilient)
146+ # RUN set -eux; \
147+ # curl -fSL --retry 5 --retry-connrefused --retry-delay 3 \
148+ # -o qualimap_v2.3.zip \
149+ # https://bitbucket.org/kokonech/qualimap/downloads/qualimap_v2.3.zip \
150+ # || curl -fSL --retry 5 --retry-connrefused --retry-delay 3 \
151+ # -o qualimap_v2.3.zip \
152+ # http://qualimap.conesalab.org/qualimap_v2.3.zip; \
153+ # unzip -q qualimap_v2.3.zip; \
154+ # mv qualimap_v2.3 /opt/qualimap; \
155+ # printf '%s\n' '#!/bin/bash' 'exec java -Xms32m -Xmx2g -jar /opt/qualimap/qualimap.jar "$@"' > /usr/local/bin/qualimap; \
156+ # chmod +x /usr/local/bin/qualimap; \
157+ # rm -f qualimap_v2.3.zip
158158
159159# create a symlink for python as hisat2 hashbangs are set for python and not python3
160160RUN ln -s /usr/bin/python3 /usr/bin/python
161161
162162# install R packages
163- # RUN Rscript -e 'install.packages("BiocManager", repos = "https://cloud.r-project.org")' \
164- # && Rscript -e 'BiocManager::install("limma")' \
165- # && Rscript -e 'BiocManager::install("edgeR")' \
166- # && Rscript -e 'BiocManager::install("DESeq2")' \
167- # && Rscript -e 'install.packages("beeswarm")' \
168- # && Rscript -e 'install.packages("knitr")' \
169- # && Rscript -e 'install.packages("gplots")' \
170- # && Rscript -e 'BiocManager::install("goseq")' \
171- # && Rscript -e 'BiocManager::install("GO.db")' \
172- # && Rscript -e 'BiocManager::install("org.Sc.sgd.db")' \
173- # && Rscript -e 'install.packages("ggiraph")' \
174- # && Rscript -e 'install.packages("plotly")'
175-
176- # # confirm all R packages load
177- # RUN Rscript -e 'library(BiocManager)' \
178- # && Rscript -e 'library(limma)' \
179- # && Rscript -e 'library(edgeR)' \
180- # && Rscript -e 'library(DESeq2)' \
181- # && Rscript -e 'library(beeswarm)' \
182- # && Rscript -e 'library(knitr)' \
183- # && Rscript -e 'library(gplots)' \
184- # && Rscript -e 'library(goseq)' \
185- # && Rscript -e 'library(GO.db)' \
186- # && Rscript -e 'library(org.Sc.sgd.db)' \
187- # && Rscript -e 'library(ggiraph)' \
188- # && Rscript -e 'library(plotly)'
163+ RUN Rscript -e 'install.packages("BiocManager", repos = "https://cloud.r-project.org")' \
164+ && Rscript -e 'BiocManager::install("limma")' \
165+ && Rscript -e 'BiocManager::install("edgeR")' \
166+ && Rscript -e 'BiocManager::install("DESeq2")' \
167+ && Rscript -e 'install.packages("beeswarm")' \
168+ && Rscript -e 'install.packages("knitr")' \
169+ && Rscript -e 'install.packages("gplots")' \
170+ && Rscript -e 'BiocManager::install("goseq")' \
171+ && Rscript -e 'BiocManager::install("GO.db")' \
172+ && Rscript -e 'BiocManager::install("org.Sc.sgd.db")' \
173+ && Rscript -e 'install.packages("ggiraph")' \
174+ && Rscript -e 'install.packages("plotly")'
175+
176+ # confirm all R packages load
177+ RUN Rscript -e 'library(BiocManager)' \
178+ && Rscript -e 'library(limma)' \
179+ && Rscript -e 'library(edgeR)' \
180+ && Rscript -e 'library(DESeq2)' \
181+ && Rscript -e 'library(beeswarm)' \
182+ && Rscript -e 'library(knitr)' \
183+ && Rscript -e 'library(gplots)' \
184+ && Rscript -e 'library(goseq)' \
185+ && Rscript -e 'library(GO.db)' \
186+ && Rscript -e 'library(org.Sc.sgd.db)' \
187+ && Rscript -e 'library(ggiraph)' \
188+ && Rscript -e 'library(plotly)'
189189
190190# Set up environment variables for the virtual environment
191191ENV PATH="/opt/venv/bin:$PATH"
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