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Expand file tree Collapse file tree Original file line number Diff line number Diff line change @@ -80,6 +80,14 @@ RUN python3 -m venv /opt/venv && \
8080 ln -s /opt/venv/bin/multiqc /usr/local/bin/ && \
8181 ln -s /opt/venv/bin/cutadapt /usr/local/bin/
8282
83+ # Install FastQC
84+ RUN wget https://www.bioinformatics.babraham.ac.uk/projects/fastqc/fastqc_v0.12.1.zip && \
85+ unzip fastqc_v0.12.1.zip && \
86+ mv FastQC /opt/ && \
87+ chmod +x /opt/FastQC/fastqc && \
88+ ln -s /opt/FastQC/fastqc /usr/local/bin/fastqc && \
89+ rm fastqc_v0.12.1.zip
90+
8391# install R packages
8492RUN Rscript -e 'install.packages("BiocManager", repos = "https://cloud.r-project.org")' \
8593 && Rscript -e 'BiocManager::install("limma")' \
@@ -108,5 +116,8 @@ RUN Rscript -e 'library(BiocManager)' \
108116 && Rscript -e 'library(ggiraph)' \
109117 && Rscript -e 'library(plotly)'
110118
119+ # Set up environment variables for the virtual environment
120+ ENV PATH="/opt/venv/bin:$PATH"
121+
111122# copy data
112123COPY docker/RNA_seq/ /var/lib/RNA_seq/
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