diff --git a/instrumentation-security/graalvm-jsinjection-24.1.0/build.gradle b/instrumentation-security/graalvm-jsinjection-24.1.0/build.gradle
new file mode 100644
index 000000000..33c3f4a6d
--- /dev/null
+++ b/instrumentation-security/graalvm-jsinjection-24.1.0/build.gradle
@@ -0,0 +1,38 @@
+dependencies {
+    implementation(project(":newrelic-security-api"))
+    implementation("com.newrelic.agent.java:newrelic-api:${nrAPIVersion}")
+    implementation("com.newrelic.agent.java:newrelic-weaver-api:${nrAPIVersion}")
+    implementation("org.graalvm.truffle:truffle-api:24.1.0")
+    implementation("org.graalvm.js:js:24.1.0")
+}
+
+jar {
+    manifest { attributes 'Implementation-Title': 'com.newrelic.instrumentation.security.graalvm-jsinjection-24.1.0' }
+}
+
+verifyInstrumentation {
+    passes 'org.graalvm.truffle:truffle-api:[24.1.0,)'
+}
+
+java {
+    toolchain {
+        languageVersion.set(JavaLanguageVersion.of(17))
+    }
+}
+
+compileJava {
+    options.fork = true
+    options.bootstrapClasspath = null
+}
+
+test {
+    // These instrumentation tests only run on Java 17+ regardless of the -PtestN gradle property that is set.
+    onlyIf {
+        !project.hasProperty('test8') && !project.hasProperty('test11')
+    }
+}
+
+site {
+    title 'JSInjection'
+    type 'Messaging'
+}
\ No newline at end of file
diff --git a/instrumentation-security/graalvm-jsinjection-24.1.0/src/main/java/com/newrelic/agent/security/instrumentation/graalvm24/JSEngineUtils.java b/instrumentation-security/graalvm-jsinjection-24.1.0/src/main/java/com/newrelic/agent/security/instrumentation/graalvm24/JSEngineUtils.java
new file mode 100644
index 000000000..457cbc28e
--- /dev/null
+++ b/instrumentation-security/graalvm-jsinjection-24.1.0/src/main/java/com/newrelic/agent/security/instrumentation/graalvm24/JSEngineUtils.java
@@ -0,0 +1,9 @@
+package com.newrelic.agent.security.instrumentation.graalvm24;
+
+public class JSEngineUtils {
+
+    public static final String NR_SEC_CUSTOM_ATTRIB_NAME = "JSENGINE_OPERATION_LOCK_NASHORN-";
+    public static final String GRAALVM_JS_INJECTION_24_1_0 = "GRAALVM_JS_INJECTION_24.1.0";
+    public static final String METHOD_EVAL = "eval";
+    public static final CharSequence LANGUAGE_ID_JS = "js";
+}
diff --git a/instrumentation-security/graalvm-jsinjection-24.1.0/src/main/java/com/oracle/truffle/polyglot/PolyglotContextImpl_Instrumentation.java b/instrumentation-security/graalvm-jsinjection-24.1.0/src/main/java/com/oracle/truffle/polyglot/PolyglotContextImpl_Instrumentation.java
new file mode 100644
index 000000000..3a36f5442
--- /dev/null
+++ b/instrumentation-security/graalvm-jsinjection-24.1.0/src/main/java/com/oracle/truffle/polyglot/PolyglotContextImpl_Instrumentation.java
@@ -0,0 +1,100 @@
+package com.oracle.truffle.polyglot;
+
+import com.newrelic.agent.security.instrumentation.graalvm24.JSEngineUtils;
+import com.newrelic.api.agent.security.NewRelicSecurity;
+import com.newrelic.api.agent.security.instrumentation.helpers.GenericHelper;
+import com.newrelic.api.agent.security.schema.AbstractOperation;
+import com.newrelic.api.agent.security.schema.StringUtils;
+import com.newrelic.api.agent.security.schema.VulnerabilityCaseType;
+import com.newrelic.api.agent.security.schema.exceptions.NewRelicSecurityException;
+import com.newrelic.api.agent.security.schema.operation.JSInjectionOperation;
+import com.newrelic.api.agent.security.utils.logging.LogLevel;
+import com.newrelic.api.agent.weaver.Weave;
+import com.newrelic.api.agent.weaver.Weaver;
+import com.oracle.truffle.api.source.Source;
+import org.graalvm.polyglot.impl.AbstractPolyglotImpl;
+
+@Weave(originalName = "com.oracle.truffle.polyglot.PolyglotContextImpl")
+final class PolyglotContextImpl_Instrumentation {
+
+    public AbstractPolyglotImpl.APIAccess getAPIAccess() {
+        return Weaver.callOriginal();
+    }
+
+    public Object eval(String languageId, Object source) {
+        boolean isLockAcquired = acquireLockIfPossible();
+        AbstractOperation operation = null;
+        if (isLockAcquired) {
+            operation = preprocessSecurityHook(languageId, source, JSEngineUtils.METHOD_EVAL);
+        }
+        Object result;
+        try {
+            result = Weaver.callOriginal();
+        } finally {
+            if (isLockAcquired) {
+                releaseLock();
+            }
+        }
+        registerExitOperation(isLockAcquired, operation);
+        return result;
+    }
+
+    private void registerExitOperation(boolean isProcessingAllowed, AbstractOperation operation) {
+        try {
+            if (operation == null || !isProcessingAllowed || !NewRelicSecurity.isHookProcessingActive() ||
+                    NewRelicSecurity.getAgent().getSecurityMetaData().getRequest().isEmpty() || GenericHelper.skipExistsEvent()
+            ) {
+                return;
+            }
+            NewRelicSecurity.getAgent().registerExitEvent(operation);
+        } catch (Throwable ignored) {
+            NewRelicSecurity.getAgent()
+                    .log(LogLevel.FINEST,
+                            String.format(GenericHelper.EXIT_OPERATION_EXCEPTION_MESSAGE, JSEngineUtils.GRAALVM_JS_INJECTION_24_1_0, ignored.getMessage()),
+                            ignored, this.getClass().getName());
+        }
+    }
+
+    private AbstractOperation preprocessSecurityHook(String languageId, Object source, String methodName) {
+        try {
+            if (!NewRelicSecurity.isHookProcessingActive() || NewRelicSecurity.getAgent().getSecurityMetaData().getRequest().isEmpty() ||
+                    !StringUtils.equalsIgnoreCase(languageId, JSEngineUtils.LANGUAGE_ID_JS)) {
+                return null;
+            }
+
+            // Getting the source to get the Characters
+            Source sourceReceiver = (com.oracle.truffle.api.source.Source) getAPIAccess().getSourceReceiver(source);
+            JSInjectionOperation jsInjectionOperation = new JSInjectionOperation(String.valueOf(sourceReceiver.getCharacters()), this.getClass().getName(),
+                    methodName);
+            NewRelicSecurity.getAgent().registerOperation(jsInjectionOperation);
+            return jsInjectionOperation;
+        } catch (Throwable e) {
+            if (e instanceof NewRelicSecurityException) {
+                NewRelicSecurity.getAgent()
+                        .log(LogLevel.WARNING,
+                                String.format(GenericHelper.SECURITY_EXCEPTION_MESSAGE, JSEngineUtils.GRAALVM_JS_INJECTION_24_1_0, e.getMessage()), e,
+                                this.getClass().getName());
+                throw e;
+            }
+            NewRelicSecurity.getAgent()
+                    .log(LogLevel.SEVERE,
+                            String.format(GenericHelper.REGISTER_OPERATION_EXCEPTION_MESSAGE, JSEngineUtils.GRAALVM_JS_INJECTION_24_1_0, e.getMessage()), e,
+                            this.getClass().getName());
+            NewRelicSecurity.getAgent()
+                    .reportIncident(LogLevel.SEVERE,
+                            String.format(GenericHelper.REGISTER_OPERATION_EXCEPTION_MESSAGE, JSEngineUtils.GRAALVM_JS_INJECTION_24_1_0, e.getMessage()), e,
+                            this.getClass().getName());
+        }
+        return null;
+    }
+
+    private void releaseLock() {
+        GenericHelper.releaseLock(JSEngineUtils.NR_SEC_CUSTOM_ATTRIB_NAME);
+    }
+
+    private boolean acquireLockIfPossible() {
+        return GenericHelper.acquireLockIfPossible(VulnerabilityCaseType.JAVASCRIPT_INJECTION, JSEngineUtils.NR_SEC_CUSTOM_ATTRIB_NAME);
+    }
+
+}
+
diff --git a/settings.gradle b/settings.gradle
index 64dd79da8..28ae0a5e2 100644
--- a/settings.gradle
+++ b/settings.gradle
@@ -107,6 +107,7 @@ include 'instrumentation:nashorn-jsinjection'
 include 'instrumentation:rhino-jsinjection'
 include 'instrumentation:graalvm-jsinjection-19.0.0'
 include 'instrumentation:graalvm-jsinjection-22.0.0'
+include 'instrumentation:graalvm-jsinjection-24.1.0'
 include 'instrumentation:apache-log4j-2.0'
 include 'instrumentation:apache-log4j-2.17.2'
 include 'instrumentation:apache-log4j-3.0.0'