- Generated: 2026-01-16T10:29:59.227134598Z
- Nextflow version: 25.12.0-edge
- Summary: 11 errors, 74 warnings
-
Error:
modules/local/format_fasta_mirna/main.nf:1:1: Statements cannot be mixed with script declarations -- move statements into a process, workflow, or functiondef VERSION = '0.0.14' ^^^^^^^^^^^^^^^^^^^^^^
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Error:
modules/local/format_fasta_mirna/main.nf:28:34:VERSIONis not definedfastx_toolkit: \$(echo "$VERSION") ^^^^^^^^
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Error:
subworkflows/local/prepare_genome/main.nf:24:5:forloops are no longer supportedfor (file_path in files) { ^^^
-
Error:
subworkflows/local/prepare_genome/main.nf:24:10:file_pathis not definedfor (file_path in files) { ^^^^^^^^^
-
Error:
subworkflows/local/prepare_genome/main.nf:25:25:file_pathis not defineddef file_name = file_path.getName() ^^^^^^^^^
-
Error:
subworkflows/local/prepare_genome/main.nf:28:13:breakis not allowed as an identifier because it is a Groovy keywordbreak ^^^^^ -
Error:
subworkflows/local/prepare_genome/main.nf:28:13:breakis not definedbreak ^^^^^ -
Error:
workflows/smrnaseq.nf:34:1: Statements cannot be mixed with script declarations -- move statements into a process, workflow, or functionch_multiqc_config = channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
-
Error:
workflows/smrnaseq.nf:35:1: Statements cannot be mixed with script declarations -- move statements into a process, workflow, or functionch_multiqc_custom_config = params.multiqc_config ? channel.fromPath( params.multiqc_config, checkIfExists: true ) : channel.empty() ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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Error:
workflows/smrnaseq.nf:36:1: Statements cannot be mixed with script declarations -- move statements into a process, workflow, or functionch_multiqc_logo = params.multiqc_logo ? channel.fromPath( params.multiqc_logo, checkIfExists: true ) : channel.empty() ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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Error:
workflows/smrnaseq.nf:37:1: Statements cannot be mixed with script declarations -- move statements into a process, workflow, or functionch_fastp_adapters = channel.fromPath(params.fastp_known_mirna_adapters, checkIfExists: true).collect() // collect to consume for all incoming samples to FASTP ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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Warning:
modules/local/format_fasta_mirna/main.nf:1:5: Variable was declared but not useddef VERSION = '0.0.14' ^^^^^^^
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Warning:
modules/nf-core/blat/main.nf:52:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/cat/fastq/main.nf:22:60: Implicit closure parameter is deprecated, declare an explicit parameter insteaddef readList = reads instanceof List ? reads.collect { it.toString() } : [reads.toString()] ^^
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Warning:
modules/nf-core/cat/fastq/main.nf:58:60: Implicit closure parameter is deprecated, declare an explicit parameter insteaddef readList = reads instanceof List ? reads.collect { it.toString() } : [reads.toString()] ^^
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Warning:
modules/nf-core/mirdeep2/mapper/main.nf:41:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/mirdeep2/mirdeep2/main.nf:51:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/mirtop/counts/main.nf:24:9: Variable was declared but not useddef prefix = task.ext.prefix ?: "${meta.id}" ^^^^^^
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Warning:
modules/nf-core/mirtop/counts/main.nf:42:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/mirtop/counts/main.nf:43:9: Variable was declared but not useddef prefix = task.ext.prefix ?: "${meta.id}" ^^^^^^
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Warning:
modules/nf-core/mirtop/export/main.nf:26:9: Variable was declared but not useddef prefix = task.ext.prefix ?: "${meta.id}" ^^^^^^
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Warning:
modules/nf-core/mirtop/export/main.nf:44:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/mirtop/gff/main.nf:44:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/mirtop/gff/main.nf:45:9: Variable was declared but not useddef prefix = task.ext.prefix ?: "${meta.id}" ^^^^^^
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Warning:
modules/nf-core/mirtop/stats/main.nf:24:9: Variable was declared but not useddef prefix = task.ext.prefix ?: "${meta.id}" ^^^^^^
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Warning:
modules/nf-core/mirtop/stats/main.nf:39:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/mirtrace/qc/main.nf:26:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/mirtrace/qc/main.nf:27:9: Variable was declared but not useddef prefix = task.ext.prefix ?: "${meta.id}" ^^^^^^
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Warning:
modules/nf-core/mirtrace/qc/main.nf:45:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/seqcluster/collapse/main.nf:41:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
modules/nf-core/seqkit/grep/main.nf:45:9: Variable was declared but not useddef args = task.ext.args ?: '' ^^^^
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Warning:
subworkflows/local/contaminant_filter/main.nf:59:5: Variable was declared but not usedch_mqc_results = channel.empty() ^^^^^^^^^^^^^^
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Warning:
subworkflows/local/contaminant_filter/main.nf:135:24: Parameter was not used -- prefix with_to suppress warning.map { meta, file -> file.text.readLines() } ^^^^ -
Warning:
subworkflows/local/contaminant_filter/main.nf:181:24: Parameter was not used -- prefix with_to suppress warning.map { meta, file -> file.text.readLines() } ^^^^ -
Warning:
subworkflows/local/contaminant_filter/main.nf:227:24: Parameter was not used -- prefix with_to suppress warning.map { meta, file -> file.text.readLines() } ^^^^ -
Warning:
subworkflows/local/contaminant_filter/main.nf:273:24: Parameter was not used -- prefix with_to suppress warning.map { meta, file -> file.text.readLines() } ^^^^ -
Warning:
subworkflows/local/mirna_quant/main.nf:53:27: Implicit closure parameter is deprecated, declare an explicit parameter instead.map { add_suffix(it, "mature") } ^^ -
Warning:
subworkflows/local/mirna_quant/main.nf:59:27: Implicit closure parameter is deprecated, declare an explicit parameter instead.map { add_suffix(it, "hairpin") } ^^ -
Warning:
subworkflows/local/mirna_quant/main.nf:81:60: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_edger_input = BAM_STATS_MATURE.out.idxstats.collect{it[1]} ^^
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Warning:
subworkflows/local/mirna_quant/main.nf:82:53: Implicit closure parameter is deprecated, declare an explicit parameter instead.mix(BAM_STATS_HAIRPIN.out.idxstats.collect{it[1]}) ^^
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Warning:
subworkflows/local/mirna_quant/main.nf:106:5: Variable was declared but not usedch_tsvs = BAM_STATS_MIRNA_MIRTOP.out.counts ^^^^^^^
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Warning:
subworkflows/local/mirna_quant/main.nf:107:18: Implicit closure parameter is deprecated, declare an explicit parameter instead.collect{it[1]} ^^ -
Warning:
subworkflows/local/mirna_quant/main.nf:114:16: Parameter was not used -- prefix with_to suppress warning.map { meta, file -> file } ^^^^ -
Warning:
subworkflows/local/mirna_quant/main.nf:127:27: Implicit closure parameter is deprecated, declare an explicit parameter instead.map { add_suffix(it, "genome") } ^^ -
Warning:
subworkflows/local/prepare_genome/main.nf:98:27: Parameter was not used -- prefix with_to suppress warning.map{ meta, index_dir -> ^^^^ -
Warning:
subworkflows/local/utils_nfcore_smrnaseq_pipeline/main.nf:34:5: Parameter was not used -- prefix with_to suppress warningmonochrome_logs // boolean: Do not use coloured log outputs ^^^^^^^^^^^^^^^ -
Warning:
subworkflows/local/utils_nfcore_smrnaseq_pipeline/main.nf:37:5: Parameter was not used -- prefix with_to suppress warninginput // string: Path to input samplesheet ^^^^^ -
Warning:
subworkflows/local/utils_nfcore_smrnaseq_pipeline/main.nf:246:35: Implicit closure parameter is deprecated, declare an explicit parameter insteadfastqs = fastqs.collect { it.take(1) } ^^
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Warning:
subworkflows/nf-core/bam_sort_stats_samtools/main.nf:16:19: The use ofChannelto access channel factories is deprecated -- usechannelinsteadch_versions = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/bam_stats_mirna_mirtop/main.nf:16:19: The use ofChannelto access channel factories is deprecated -- usechannelinsteadch_versions = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/bam_stats_samtools/main.nf:15:19: The use ofChannelto access channel factories is deprecated -- usechannelinsteadch_versions = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:33:22: Parameter was not used -- prefix with_to suppress warningcatch (Exception ex) { ^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:51:19: The use ofChannelto access channel factories is deprecated -- usechannelinsteadch_versions = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:52:23: The use ofChannelto access channel factories is deprecated -- usechannelinsteadfastqc_raw_html = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:53:22: The use ofChannelto access channel factories is deprecated -- usechannelinsteadfastqc_raw_zip = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:54:15: The use ofChannelto access channel factories is deprecated -- usechannelinsteadumi_log = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:55:17: The use ofChannelto access channel factories is deprecated -- usechannelinsteadtrim_json = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:56:17: The use ofChannelto access channel factories is deprecated -- usechannelinsteadtrim_html = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:57:16: The use ofChannelto access channel factories is deprecated -- usechannelinsteadtrim_log = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:58:23: The use ofChannelto access channel factories is deprecated -- usechannelinsteadtrim_reads_fail = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:59:25: The use ofChannelto access channel factories is deprecated -- usechannelinsteadtrim_reads_merged = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:60:24: The use ofChannelto access channel factories is deprecated -- usechannelinsteadfastqc_trim_html = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:61:23: The use ofChannelto access channel factories is deprecated -- usechannelinsteadfastqc_trim_zip = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:62:23: The use ofChannelto access channel factories is deprecated -- usechannelinsteadtrim_read_count = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:63:19: The use ofChannelto access channel factories is deprecated -- usechannelinsteadadapter_seq = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:66:49: Parameter was not used -- prefix with_to suppress warningreads_only = reads.map { meta, reads_files, adapter_fasta -> [ meta, reads_files ] } ^^^^^^^^^^^^^
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Warning:
subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf:104:20: Parameter was not used -- prefix with_to suppress warning.map { sample_id, meta, umi_reads_files, adapter_fasta -> [meta, umi_reads_files, adapter_fasta] } ^^^^^^^^^ -
Warning:
subworkflows/nf-core/fastq_find_mirna_mirdeep2/main.nf:16:19: The use ofChannelto access channel factories is deprecated -- usechannelinsteadch_versions = Channel.empty() ^^^^^^^
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Warning:
subworkflows/nf-core/utils_nfcore_pipeline/main.nf:101:98: The use ofChannelto access channel factories is deprecated -- usechannelinsteadreturn ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML())) ^^^^^^^
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Warning:
workflows/smrnaseq.nf:34:1: Variable was declared but not usedch_multiqc_config = channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) ^^^^^^^^^^^^^^^^^
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Warning:
workflows/smrnaseq.nf:35:1: Variable was declared but not usedch_multiqc_custom_config = params.multiqc_config ? channel.fromPath( params.multiqc_config, checkIfExists: true ) : channel.empty() ^^^^^^^^^^^^^^^^^^^^^^^^
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Warning:
workflows/smrnaseq.nf:36:1: Variable was declared but not usedch_multiqc_logo = params.multiqc_logo ? channel.fromPath( params.multiqc_logo, checkIfExists: true ) : channel.empty() ^^^^^^^^^^^^^^^
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Warning:
workflows/smrnaseq.nf:37:1: Variable was declared but not usedch_fastp_adapters = channel.fromPath(params.fastp_known_mirna_adapters, checkIfExists: true).collect() // collect to consume for all incoming samples to FASTP ^^^^^^^^^^^^^^^^^
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Warning:
workflows/smrnaseq.nf:295:9: Variable was declared but not usedch_multiqc_custom_methods_description = params.multiqc_methods_description ? ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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Warning:
workflows/smrnaseq.nf:314:104: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:315:105: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:316:99: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{it[1]}.ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:318:87: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(UMICOLLAPSE_FASTQ.out.log.collect{it[1]}.ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:321:71: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:322:87: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:323:88: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.hairpin_stats.collect({it[1]}).ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:324:86: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mirtop_logs.collect({it[1]}).ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:326:83: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(MIRTRACE_QC.out.html.collect({it[1]}).ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:327:83: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(MIRTRACE_QC.out.json.collect({it[1]}).ifEmpty([])) ^^
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Warning:
workflows/smrnaseq.nf:328:82: Implicit closure parameter is deprecated, declare an explicit parameter insteadch_multiqc_files = ch_multiqc_files.mix(MIRTRACE_QC.out.tsv.collect({it[1]}).ifEmpty([])) ^^