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$ NXF_SYNTAX_PARSER=v2 nextflow run . --help
N E X T F L O W ~ version 26.02.0-edge
Downloading plugin nf-core-utils@0.4.0
Downloading plugin nf-schema@2.6.1
Launching `./main.nf` [chaotic_austin] revision: e9312db940
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,--./,-.
___ __ __ __ ___ /,-._.--~'
|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'
nf-core/seqinspector 1.1.0dev
------------------------------------------------------
Typical pipeline command:
nextflow run nf-core/seqinspector -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>
help message of that parameter will be printed.
or `--helpFull`.
Input/output options
--input [string] Path to comma-separated file containing information about the samples in the experiment.
--sample_size [number] Take a subset of reads for analysis. [default: 0]
--outdir [string] The output directory where the results will be saved. You have to use absolute paths to storage on
Cloud infrastructure.
--email [string] Email address for completion summary.
--multiqc_title [string] MultiQC report title. Printed as page header, used for filename if not otherwise specified.
--tools [string] Comma-separated string of tools to run
--tools_bundle [string] Select some default setup for the tools to be run, tools can still be used to add tools
[default: default]
--skip_tools [string] Comma-separated string of tools to skip - overrides any other means of tools selection
Validation options
--fq_lint_args [string] Arguments to pass to FQ lint
--continue_with_lint_fail [boolean] Whether to continue with the pipeline if linting fails for a single sample. [default: true]
--checkqc_config [string] Path to custom CheckQC config
Kraken2 options
--kraken2_db [string] Path to Kraken2 database file, either a gzipped file or the path to the uncompressed database.
--kraken2_save_reads [boolean] Turn on saving of Kraken2-aligned reads
--kraken2_save_readclassifications [boolean] Turn on saving of Kraken2 per-read taxonomic assignment file
--kraken_save_uncompressed_db [boolean] Turn on saving of uncompressed Kraken2 DB
Reference genome options
--genome [string] Name of iGenomes reference.
--fastq_screen_references [string] A .csv of reference genomes to be mapped against by FastQ Screen [default:
${projectDir}/assets/example_fastq_screen_references.csv]
--fasta [string] Path to FASTA genome file.
--bwamem2 [string] Path to bwamem2 indices.
--dict [string] Sequence dictionary for the reference fasta file provided.
--fai [string] Fasta index for the reference fasta file provided.
--target_intervals [string] Path to target intervals file.
--bait_intervals [string] Path to bait intervals file.
Generic options
--multiqc_methods_description [string] Custom MultiQC yaml file containing HTML including a methods description.
--help [boolean, string] Display the help message.
--help_full [boolean] Display the full detailed help message.
--show_hidden [boolean] Display hidden parameters in the help message (only works when --help or --help_full are provided).
!! Hiding 20 param(s), use the `--showHidden` parameter to show them !!
------------------------------------------------------
* The pipeline
https://doi.org/10.5281/zenodo.18757486
* The nf-core framework
https://doi.org/10.1038/s41587-020-0439-x
* Software dependencies
https://github.com/nf-core/seqinspector/blob/master/CITATIONS.md