diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9988dfc4f9..df29490da9 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -69,6 +69,7 @@
 - Include AI and LLM usage guidelines in CONTRIBUTING.md ([#4211](https://github.com/nf-core/tools/pull/4211))
 - Update conda-incubator/setup-miniconda action to v4 ([#4215](https://github.com/nf-core/tools/pull/4215))
 - Update dependency textual to v8.2.4 ([#4217](https://github.com/nf-core/tools/pull/4217))
+- Update python:3.14-slim Docker digest to 5b3879b ([#4221](https://github.com/nf-core/tools/pull/4221))
 
 ### Download
 
diff --git a/Dockerfile b/Dockerfile
index c742b0611d..91e6e56ca7 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM python:3.14-slim@sha256:bc389f7dfcb21413e72a28f491985326994795e34d2b86c8ae2f417b4e7818aa
+FROM python:3.14-slim@sha256:5b3879b6f3cb77e712644d50262d05a7c146b7312d784a18eff7ff5462e77033
 LABEL authors="phil.ewels@seqera.io,erik.danielsson@scilifelab.se" \
     description="Docker image containing requirements for nf-core/tools"