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Optionally use host-filtered reads in downstream mapping steps #281

@Joon-Klaps

Description

@Joon-Klaps

Description of feature

Add an opt-in parameter to use host-filtered or host-depleted FASTQ files for downstream mapping and polishing steps when those files are available.

See original proposal in #280

Current behavior

Downstream steps such as sspace_basic, bwamem2, and bowtie2 currently use the original clean FASTQ files. This preserves current behavior, but it can increase runtime and storage usage substantially on large datasets.

Proposal

  • Add a new pipeline parameter, defaulting to false.
  • When enabled, prefer host-filtered reads for downstream mapping and polishing steps after host subtraction has completed successfully.
  • Keep the current clean-read behavior as the default for backwards compatibility.
  • Update tests and documentation accordingly.

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