@@ -16,49 +16,49 @@ editorial_board:
1616- subinamehta
1717
1818pathway :
19- - section : " Module 1 : Fusion-Database Generation"
19+ - section : " Module 1a : Fusion-Database Generation"
2020 description : |
2121 Learn how to generate a personalized fusion peptide database using RNA-seq data. This step sets the foundation for identifying tumor-specific fusion peptides in downstream analyses.
2222 tutorials :
2323 - name : neoantigen-1-fusion-database-generation
2424 topic : proteomics
2525
26- - section : " Module 2 : Non-Reference Database Generation"
26+ - section : " Module 1b : Non-Reference Database Generation"
2727 description : |
2828 Construct a non-reference proteogenomic database incorporating somatic mutations, indels, and other genomic alterations from VCF data.
2929 tutorials :
3030 - name : neoantigen-2-non-reference-database-generation
3131 topic : proteomics
3232
33- - section : " Module 3 : Database Merge and FragPipe Discovery"
33+ - section : " Module 2 : Database Merge and FragPipe Discovery"
3434 description : |
3535 Merge the fusion and non-reference databases, then use FragPipe for mass spectrometry-based discovery of putative neopeptides.
3636 tutorials :
3737 - name : neoantigen-3-fragpipe-discovery
3838 topic : proteomics
3939
40- - section : " Module 4 : PepQuery2 Verification"
40+ - section : " Module 3 : PepQuery2 Verification"
4141 description : |
4242 Perform targeted verification of neoantigen candidates using PepQuery2 for peptide-spectrum match validation.
4343 tutorials :
4444 - name : neoantigen-4-peptide-verification
4545 topic : proteomics
4646
47- - section : " Module 5 : Variant Annotation"
47+ - section : " Module 4 : Variant Annotation"
4848 description : |
4949 Annotate validated neopeptides with their corresponding genomic variants and protein context.
5050 tutorials :
5151 - name : neoantigen-5-variant-annotation
5252 topic : proteomics
5353
54- - section : " Module 6 : Predicting HLA Binding"
54+ - section : " Module 5a : Predicting HLA Binding"
5555 description : |
5656 Predict MHC binding affinity of validated neopeptides using tools such as NetMHCpan or similar.
5757 tutorials :
5858 - name : neoantigen-6-predicting-hla-binding
5959 topic : proteomics
6060
61- - section : " Module 7 : IEDB Binding of PepQuery Validated Neopeptides"
61+ - section : " Module 5b : IEDB Binding of PepQuery Validated Neopeptides"
6262 description : |
6363 Assess the immunogenic potential of neopeptides by checking their binding predictions against immune epitope databases such as IEDB.
6464 tutorials :
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