diff --git a/boot/zephyr/include/nrf_cleanup.h b/boot/zephyr/include/nrf_cleanup.h
index 8cd8fe3774..dfd9249ffc 100644
--- a/boot/zephyr/include/nrf_cleanup.h
+++ b/boot/zephyr/include/nrf_cleanup.h
@@ -11,8 +11,8 @@
  * Perform cleanup on some peripheral resources used by MCUBoot prior chainload
  * the application.
  *
- * This function disables all RTC instances and UARTE instances.
- * It Disables their interrupts signals as well.
+ * This function disables all RTC, UARTE, and SPIM instances.
+ * It disables their interrupts signals as well.
  */
 void nrf_cleanup_peripheral(void);
 
diff --git a/boot/zephyr/nrf_cleanup.c b/boot/zephyr/nrf_cleanup.c
index 0177f58371..56c4550f38 100644
--- a/boot/zephyr/nrf_cleanup.c
+++ b/boot/zephyr/nrf_cleanup.c
@@ -26,7 +26,9 @@
     #include <hal/nrf_vpr.h>
     #include <softperipheral_regif.h>
 #endif
-
+#if defined(SPIM_PRESENT)
+    #include <hal/nrf_spim.h>
+#endif
 
 #include <string.h>
 
@@ -200,6 +202,33 @@ void nrf_cleanup_peripheral(void)
     }
 #endif
 
+#if defined(SPIM_PRESENT)
+    /* Stop SPIM EasyDMA and disable all instances to prevent stale DMA
+     * from causing RAMACCERR when TF-M reconfigures SPU permissions. */
+    {
+        NRF_SPIM_Type *spim_inst[] = {
+#if defined(NRF_SPIM0)
+            NRF_SPIM0,
+#endif
+#if defined(NRF_SPIM1)
+            NRF_SPIM1,
+#endif
+#if defined(NRF_SPIM2)
+            NRF_SPIM2,
+#endif
+#if defined(NRF_SPIM3)
+            NRF_SPIM3,
+#endif
+        };
+
+        for (int i = 0; i < sizeof(spim_inst) / sizeof(spim_inst[0]); ++i) {
+            nrf_spim_task_trigger(spim_inst[i], NRF_SPIM_TASK_STOP);
+            nrf_spim_disable(spim_inst[i]);
+            nrf_spim_int_disable(spim_inst[i], 0xFFFFFFFF);
+        }
+    }
+#endif
+
 #if defined(NRF_PPI)
     nrf_ppi_channels_disable_all(NRF_PPI);
 #endif
diff --git a/docs/release-notes.d/spim-cleanup.md b/docs/release-notes.d/spim-cleanup.md
new file mode 100644
index 0000000000..2de2f588fd
--- /dev/null
+++ b/docs/release-notes.d/spim-cleanup.md
@@ -0,0 +1,2 @@
+ - Added SPIM peripheral cleanup in nrf_cleanup to prevent RAMACCERR when
+   TF-M reconfigures SPU SRAM permissions after MCUboot uses SPI flash.