|
1 | 1 | { |
2 | | - "cells": [ |
3 | | - { |
4 | | - "cell_type": "markdown", |
5 | | - "id": "255d97a8", |
6 | | - "metadata": {}, |
7 | | - "source": [ |
8 | | - "# Write OME-ZARR images\n", |
9 | | - "(basic:write)=\n", |
10 | | - "\n", |
11 | | - "Writing ome-zarr images is primarily exposed through the {py:class}`ome_zarr.image.NgffImage` and {py:class}`ome_zarr.image.NgffMultiscales` classes, which provide a high-level API for creating and manipulating OME-ZARR images and pyramids." |
12 | | - ] |
13 | | - }, |
14 | | - { |
15 | | - "cell_type": "code", |
16 | | - "execution_count": 1, |
17 | | - "id": "21e12529", |
18 | | - "metadata": {}, |
19 | | - "outputs": [], |
20 | | - "source": [ |
21 | | - "import numpy as np\n", |
22 | | - "from ome_zarr.image import NgffImage, NgffMultiscales" |
23 | | - ] |
24 | | - }, |
25 | | - { |
26 | | - "cell_type": "markdown", |
27 | | - "id": "33774809", |
28 | | - "metadata": {}, |
29 | | - "source": [ |
30 | | - "Let's first create some random data to write:" |
31 | | - ] |
32 | | - }, |
33 | | - { |
34 | | - "cell_type": "code", |
35 | | - "execution_count": 2, |
36 | | - "id": "4cdd0f46", |
37 | | - "metadata": {}, |
38 | | - "outputs": [], |
39 | | - "source": [ |
40 | | - "path = \"test_ngff.ome.zarr\"\n", |
41 | | - "\n", |
42 | | - "# create some random data to write\n", |
43 | | - "size_xy = 128\n", |
44 | | - "size_z = 10\n", |
45 | | - "rng = np.random.default_rng(0)\n", |
46 | | - "data = rng.poisson(lam=10, size=(size_z, size_xy, size_xy)).astype(np.uint8)" |
47 | | - ] |
48 | | - }, |
49 | | - { |
50 | | - "cell_type": "code", |
51 | | - "execution_count": 4, |
52 | | - "id": "ce122c48", |
53 | | - "metadata": {}, |
54 | | - "outputs": [ |
| 2 | + "cells": [ |
55 | 3 | { |
56 | | - "data": { |
57 | | - "text/plain": [ |
58 | | - "[]" |
| 4 | + "cell_type": "markdown", |
| 5 | + "id": "255d97a8", |
| 6 | + "metadata": {}, |
| 7 | + "source": [ |
| 8 | + "# Write OME-ZARR images\n", |
| 9 | + "(basic:write)=\n", |
| 10 | + "\n", |
| 11 | + "Writing ome-zarr images is primarily exposed through the {py:class}`ome_zarr.image.NgffImage` and {py:class}`ome_zarr.image.NgffMultiscales` classes, which provide a high-level API for creating and manipulating OME-ZARR images and pyramids." |
59 | 12 | ] |
60 | | - }, |
61 | | - "execution_count": 4, |
62 | | - "metadata": {}, |
63 | | - "output_type": "execute_result" |
64 | | - } |
65 | | - ], |
66 | | - "source": [ |
67 | | - "ngff_image = NgffImage(\n", |
68 | | - " data=data,\n", |
69 | | - " axes=\"zyx\",\n", |
70 | | - " scale={\"z\": 0.5, \"y\": 0.1, \"x\": 0.1},\n", |
71 | | - ")\n", |
72 | | - "\n", |
73 | | - "ngff_multiscales = NgffMultiscales(image=ngff_image)\n", |
74 | | - "ngff_multiscales.to_ome_zarr(path)" |
75 | | - ] |
76 | | - }, |
77 | | - { |
78 | | - "cell_type": "markdown", |
79 | | - "id": "9015365c", |
80 | | - "metadata": {}, |
81 | | - "source": [ |
82 | | - "## Write OME-ZARR images: Legacy API\n", |
83 | | - "\n", |
84 | | - "\n", |
85 | | - "In previous versions, the principle entry-point for writing OME-ZARR images was {py:func}`ome_zarr.writer.write_image`.\n", |
86 | | - "This takes an n-dimensional `numpy` array or `dask` array and writes it to the specified `zarr group` according to the OME-ZARR specification.\n", |
87 | | - "By default, a pyramid of resolution levels will be created by down-sampling the data by a factor\n", |
88 | | - "of 2 in the X and Y dimensions.\n", |
89 | | - "For more custom control over the pyramid, see the more in-depth example on [scaling functions and scale factors](advanced:pyramid)." |
90 | | - ] |
91 | | - }, |
92 | | - { |
93 | | - "cell_type": "code", |
94 | | - "execution_count": null, |
95 | | - "id": "f87d36fa", |
96 | | - "metadata": {}, |
97 | | - "outputs": [ |
| 13 | + }, |
98 | 14 | { |
99 | | - "data": { |
100 | | - "text/plain": [ |
101 | | - "[]" |
| 15 | + "cell_type": "code", |
| 16 | + "execution_count": 1, |
| 17 | + "id": "21e12529", |
| 18 | + "metadata": {}, |
| 19 | + "outputs": [], |
| 20 | + "source": [ |
| 21 | + "import numpy as np\n", |
| 22 | + "from ome_zarr.image import NgffImage, NgffMultiscales" |
102 | 23 | ] |
103 | | - }, |
104 | | - "execution_count": 1, |
105 | | - "metadata": {}, |
106 | | - "output_type": "execute_result" |
107 | | - } |
108 | | - ], |
109 | | - "source": [ |
110 | | - "import numpy as np\n", |
111 | | - "\n", |
112 | | - "from ome_zarr.writer import write_image\n", |
113 | | - "\n", |
114 | | - "path = \"test_ngff_image.ome.zarr\"\n", |
115 | | - "write_image(data, path, axes=\"zyx\")" |
116 | | - ] |
117 | | - }, |
118 | | - { |
119 | | - "cell_type": "markdown", |
120 | | - "id": "03f9f06a", |
121 | | - "metadata": {}, |
122 | | - "source": [ |
123 | | - "Alternatively, the {py:func}`ome_zarr.writer.write_multiscale` can be used,\n", |
124 | | - "which takes a \"pyramid\" of pre-computed `numpy` arrays.\n", |
125 | | - "\n", |
126 | | - "The default version of OME-NGFF is v0.5, which is based on Zarr v3.\n", |
127 | | - "A zarr v3 group and store is created by `zarr.open_group()` below.\n", |
128 | | - "To write OME-NGFF v0.4 (Zarr v2), add the `zarr_format=2` argument." |
129 | | - ] |
130 | | - }, |
131 | | - { |
132 | | - "cell_type": "code", |
133 | | - "execution_count": null, |
134 | | - "id": "cfb1d49f", |
135 | | - "metadata": {}, |
136 | | - "outputs": [ |
| 24 | + }, |
| 25 | + { |
| 26 | + "cell_type": "markdown", |
| 27 | + "id": "33774809", |
| 28 | + "metadata": {}, |
| 29 | + "source": [ |
| 30 | + "Let's first create some random data to write:" |
| 31 | + ] |
| 32 | + }, |
| 33 | + { |
| 34 | + "cell_type": "code", |
| 35 | + "execution_count": 2, |
| 36 | + "id": "4cdd0f46", |
| 37 | + "metadata": {}, |
| 38 | + "outputs": [], |
| 39 | + "source": [ |
| 40 | + "path = \"test_ngff.ome.zarr\"\n", |
| 41 | + "\n", |
| 42 | + "# create some random data to write\n", |
| 43 | + "size_xy = 128\n", |
| 44 | + "size_z = 10\n", |
| 45 | + "rng = np.random.default_rng(0)\n", |
| 46 | + "data = rng.poisson(lam=10, size=(size_z, size_xy, size_xy)).astype(np.uint8)" |
| 47 | + ] |
| 48 | + }, |
137 | 49 | { |
138 | | - "data": { |
139 | | - "text/plain": [ |
140 | | - "[]" |
| 50 | + "cell_type": "code", |
| 51 | + "execution_count": 4, |
| 52 | + "id": "ce122c48", |
| 53 | + "metadata": {}, |
| 54 | + "outputs": [ |
| 55 | + { |
| 56 | + "data": { |
| 57 | + "text/plain": [ |
| 58 | + "[]" |
| 59 | + ] |
| 60 | + }, |
| 61 | + "execution_count": 4, |
| 62 | + "metadata": {}, |
| 63 | + "output_type": "execute_result" |
| 64 | + } |
| 65 | + ], |
| 66 | + "source": [ |
| 67 | + "ngff_image = NgffImage(\n", |
| 68 | + " data=data,\n", |
| 69 | + " axes=\"zyx\",\n", |
| 70 | + " scale={\"z\": 0.5, \"y\": 0.1, \"x\": 0.1},\n", |
| 71 | + ")\n", |
| 72 | + "\n", |
| 73 | + "ngff_multiscales = NgffMultiscales(image=ngff_image)\n", |
| 74 | + "ngff_multiscales.to_ome_zarr(path)" |
141 | 75 | ] |
142 | | - }, |
143 | | - "execution_count": 3, |
144 | | - "metadata": {}, |
145 | | - "output_type": "execute_result" |
| 76 | + }, |
| 77 | + { |
| 78 | + "cell_type": "markdown", |
| 79 | + "id": "9015365c", |
| 80 | + "metadata": {}, |
| 81 | + "source": [ |
| 82 | + "## Write OME-ZARR images: Legacy API\n", |
| 83 | + "\n", |
| 84 | + "\n", |
| 85 | + "In previous versions, the principle entry-point for writing OME-ZARR images was {py:func}`ome_zarr.writer.write_image`.\n", |
| 86 | + "This takes an n-dimensional `numpy` array or `dask` array and writes it to the specified `zarr group` according to the OME-ZARR specification.\n", |
| 87 | + "By default, a pyramid of resolution levels will be created by down-sampling the data by a factor\n", |
| 88 | + "of 2 in the X and Y dimensions.\n", |
| 89 | + "For more custom control over the pyramid, see the more in-depth example on [scaling functions and scale factors](advanced:pyramid)." |
| 90 | + ] |
| 91 | + }, |
| 92 | + { |
| 93 | + "cell_type": "code", |
| 94 | + "execution_count": null, |
| 95 | + "id": "f87d36fa", |
| 96 | + "metadata": {}, |
| 97 | + "outputs": [ |
| 98 | + { |
| 99 | + "data": { |
| 100 | + "text/plain": [ |
| 101 | + "[]" |
| 102 | + ] |
| 103 | + }, |
| 104 | + "execution_count": 1, |
| 105 | + "metadata": {}, |
| 106 | + "output_type": "execute_result" |
| 107 | + } |
| 108 | + ], |
| 109 | + "source": [ |
| 110 | + "import numpy as np\n", |
| 111 | + "\n", |
| 112 | + "from ome_zarr.writer import write_image\n", |
| 113 | + "\n", |
| 114 | + "path = \"test_ngff_image.ome.zarr\"\n", |
| 115 | + "write_image(data, path, axes=\"zyx\")" |
| 116 | + ] |
| 117 | + }, |
| 118 | + { |
| 119 | + "cell_type": "markdown", |
| 120 | + "id": "03f9f06a", |
| 121 | + "metadata": {}, |
| 122 | + "source": [ |
| 123 | + "Alternatively, the {py:func}`ome_zarr.writer.write_multiscale` can be used,\n", |
| 124 | + "which takes a \"pyramid\" of pre-computed `numpy` arrays.\n", |
| 125 | + "\n", |
| 126 | + "The default version of OME-NGFF is v0.5, which is based on Zarr v3.\n", |
| 127 | + "A zarr v3 group and store is created by `zarr.open_group()` below.\n", |
| 128 | + "To write OME-NGFF v0.4 (Zarr v2), add the `zarr_format=2` argument." |
| 129 | + ] |
| 130 | + }, |
| 131 | + { |
| 132 | + "cell_type": "code", |
| 133 | + "execution_count": null, |
| 134 | + "id": "cfb1d49f", |
| 135 | + "metadata": {}, |
| 136 | + "outputs": [ |
| 137 | + { |
| 138 | + "data": { |
| 139 | + "text/plain": [ |
| 140 | + "[]" |
| 141 | + ] |
| 142 | + }, |
| 143 | + "execution_count": 3, |
| 144 | + "metadata": {}, |
| 145 | + "output_type": "execute_result" |
| 146 | + } |
| 147 | + ], |
| 148 | + "source": [ |
| 149 | + "path = \"test_ngff_image_v2.ome.zarr\"\n", |
| 150 | + "write_image(data, path, axes=\"zyx\", zarr_format=2)" |
| 151 | + ] |
| 152 | + }, |
| 153 | + { |
| 154 | + "cell_type": "markdown", |
| 155 | + "id": "7b9a0138", |
| 156 | + "metadata": {}, |
| 157 | + "source": [ |
| 158 | + "To view the image, see tutorial on [viewing images](basic:view_images)." |
| 159 | + ] |
| 160 | + }, |
| 161 | + { |
| 162 | + "cell_type": "markdown", |
| 163 | + "id": "1f8a602d", |
| 164 | + "metadata": {}, |
| 165 | + "source": [] |
| 166 | + } |
| 167 | + ], |
| 168 | + "metadata": { |
| 169 | + "kernelspec": { |
| 170 | + "display_name": "ome-zarr (3.12.12)", |
| 171 | + "language": "python", |
| 172 | + "name": "python3" |
| 173 | + }, |
| 174 | + "language_info": { |
| 175 | + "codemirror_mode": { |
| 176 | + "name": "ipython", |
| 177 | + "version": 3 |
| 178 | + }, |
| 179 | + "file_extension": ".py", |
| 180 | + "mimetype": "text/x-python", |
| 181 | + "name": "python", |
| 182 | + "nbconvert_exporter": "python", |
| 183 | + "pygments_lexer": "ipython3", |
| 184 | + "version": "3.12.12" |
146 | 185 | } |
147 | | - ], |
148 | | - "source": [ |
149 | | - "path = \"test_ngff_image_v2.ome.zarr\"\n", |
150 | | - "write_image(data, path, axes=\"zyx\", zarr_format=2)" |
151 | | - ] |
152 | | - }, |
153 | | - { |
154 | | - "cell_type": "markdown", |
155 | | - "id": "7b9a0138", |
156 | | - "metadata": {}, |
157 | | - "source": [ |
158 | | - "To view the image, see tutorial on [viewing images](basic:view_images)." |
159 | | - ] |
160 | | - }, |
161 | | - { |
162 | | - "cell_type": "markdown", |
163 | | - "id": "1f8a602d", |
164 | | - "metadata": {}, |
165 | | - "source": [] |
166 | | - } |
167 | | - ], |
168 | | - "metadata": { |
169 | | - "kernelspec": { |
170 | | - "display_name": "ome-zarr (3.12.12)", |
171 | | - "language": "python", |
172 | | - "name": "python3" |
173 | 186 | }, |
174 | | - "language_info": { |
175 | | - "codemirror_mode": { |
176 | | - "name": "ipython", |
177 | | - "version": 3 |
178 | | - }, |
179 | | - "file_extension": ".py", |
180 | | - "mimetype": "text/x-python", |
181 | | - "name": "python", |
182 | | - "nbconvert_exporter": "python", |
183 | | - "pygments_lexer": "ipython3", |
184 | | - "version": "3.12.12" |
185 | | - } |
186 | | - }, |
187 | | - "nbformat": 4, |
188 | | - "nbformat_minor": 5 |
| 187 | + "nbformat": 4, |
| 188 | + "nbformat_minor": 5 |
189 | 189 | } |
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