diff --git a/crates/anvil-polkadot/src/api_server/server.rs b/crates/anvil-polkadot/src/api_server/server.rs
index a12f861acd951..c6ca08af41ec3 100644
--- a/crates/anvil-polkadot/src/api_server/server.rs
+++ b/crates/anvil-polkadot/src/api_server/server.rs
@@ -360,6 +360,9 @@ impl ApiServer {
             EthRequest::SetStorageAt(address, key, value) => {
                 self.set_storage_at(address, key, value).to_rpc_result()
             }
+            EthRequest::SetImmutableStorageAt(address, data) => {
+                self.set_immutable_storage_at(address, data).to_rpc_result()
+            }
             EthRequest::SetChainId(chain_id) => self.set_chain_id(chain_id).to_rpc_result(),
             // --- Revert ---
             EthRequest::EvmSnapshot(()) => self.snapshot().await.to_rpc_result(),
@@ -1327,6 +1330,49 @@ impl ApiServer {
         Ok(())
     }
 
+    fn set_immutable_storage_at(
+        &self,
+        address: Address,
+        immutables: Vec<alloy_primitives::Bytes>,
+    ) -> Result<()> {
+        node_info!("anvil_setImmutableStorageAt");
+
+        let latest_block = self.latest_block();
+
+        // Convert ABI-encoded immutable values (big-endian) to PVM format (little-endian).
+        //
+        // ## Data Format
+        //
+        // Each element in `immutables` is a single ABI-encoded immutable value:
+        // - Value in big-endian format (standard for Solidity ABI encoding)
+        // - Padded to 32 bytes
+        //
+        // Example: For a contract with immutables `uint256 value` and `address addr`:
+        //   immutables[0] = <32-byte big-endian uint256>
+        //   immutables[1] = <12 zeros + 20-byte address>
+        //
+        // ## Conversion
+        //
+        // This method converts each immutable value (32-byte chunk) from big-endian to
+        // little-endian, since PVM (Polkadot Virtual Machine) expects immutable data in
+        // little-endian format. The conversion is done by reversing each 32-byte word.
+        // Then all converted values are concatenated in order.
+        let pvm_data: Vec<u8> = immutables
+            .into_iter()
+            .flat_map(|immutable| {
+                let mut word = [0u8; 32];
+                let len = immutable.len().min(32);
+                word[..len].copy_from_slice(&immutable[..len]);
+                word.reverse();
+                word
+            })
+            .collect();
+
+        self.backend.inject_immutable_data(latest_block, address, pvm_data);
+
+        Ok(())
+    }
+
     // ----- Wallet RPCs
     async fn sign(&self, address: Address, content: impl AsRef<[u8]>) -> Result<String> {
         Ok(alloy_primitives::hex::encode_prefixed(self.wallet.sign(address, content.as_ref())?))
diff --git a/crates/anvil-polkadot/src/substrate_node/service/backend.rs b/crates/anvil-polkadot/src/substrate_node/service/backend.rs
index 9f3f256a8a355..d24257f45d8a0 100644
--- a/crates/anvil-polkadot/src/substrate_node/service/backend.rs
+++ b/crates/anvil-polkadot/src/substrate_node/service/backend.rs
@@ -207,6 +207,11 @@ impl BackendWithOverlay {
         overrides.set_code_info(at, code_hash, code_info);
     }
 
+    pub fn inject_immutable_data(&self, at: Hash, address: Address, data: Vec<u8>) {
+        let mut overrides = self.overrides.lock();
+        overrides.set_immutable_data(at, address, data);
+    }
+
     pub fn inject_child_storage(
         &self,
         at: Hash,
@@ -353,6 +358,21 @@ impl StorageOverrides {
         self.add(latest_block, changeset);
     }
 
+    fn set_immutable_data(&mut self, latest_block: Hash, address: Address, data: Vec<u8>) {
+        let mut changeset = BlockOverrides::default();
+
+        // SCALE-encode as BoundedVec<u8>: compact_length prefix followed by raw bytes
+        let mut encoded = codec::Compact(data.len() as u32).encode();
+        encoded.extend_from_slice(&data);
+
+        changeset.top.insert(
+            well_known_keys::immutable_data_of(H160::from_slice(address.as_slice())),
+            Some(encoded),
+        );
+
+        self.add(latest_block, changeset);
+    }
+
     fn set_child_storage(
         &mut self,
         latest_block: Hash,
diff --git a/crates/anvil-polkadot/src/substrate_node/service/storage.rs b/crates/anvil-polkadot/src/substrate_node/service/storage.rs
index a6553cead52c8..4f60c8c62229f 100644
--- a/crates/anvil-polkadot/src/substrate_node/service/storage.rs
+++ b/crates/anvil-polkadot/src/substrate_node/service/storage.rs
@@ -90,4 +90,13 @@ pub mod well_known_keys {
 
         key
     }
+
+    pub fn immutable_data_of(address: H160) -> Vec<u8> {
+        let mut key = Vec::new();
+        key.extend_from_slice(&twox_128("Revive".as_bytes()));
+        key.extend_from_slice(&twox_128("ImmutableDataOf".as_bytes()));
+        key.extend_from_slice(&address.encode());
+
+        key
+    }
 }
diff --git a/crates/anvil-polkadot/test-data/ImmutableStorage.json b/crates/anvil-polkadot/test-data/ImmutableStorage.json
new file mode 100644
index 0000000000000..8dec933c2ac0c
--- /dev/null
+++ b/crates/anvil-polkadot/test-data/ImmutableStorage.json
@@ -0,0 +1,75 @@
+{
+  "abi": [
+    {
+      "inputs": [
+        {
+          "internalType": "uint256",
+          "name": "_value",
+          "type": "uint256"
+        },
+        {
+          "internalType": "address",
+          "name": "_addr",
+          "type": "address"
+        }
+      ],
+      "stateMutability": "nonpayable",
+      "type": "constructor"
+    },
+    {
+      "inputs": [],
+      "name": "getImmutableAddress",
+      "outputs": [
+        {
+          "internalType": "address",
+          "name": "",
+          "type": "address"
+        }
+      ],
+      "stateMutability": "view",
+      "type": "function"
+    },
+    {
+      "inputs": [],
+      "name": "getImmutableValue",
+      "outputs": [
+        {
+          "internalType": "uint256",
+          "name": "",
+          "type": "uint256"
+        }
+      ],
+      "stateMutability": "view",
+      "type": "function"
+    },
+    {
+      "inputs": [],
+      "name": "immutableAddress",
+      "outputs": [
+        {
+          "internalType": "address",
+          "name": "",
+          "type": "address"
+        }
+      ],
+      "stateMutability": "view",
+      "type": "function"
+    },
+    {
+      "inputs": [],
+      "name": "immutableValue",
+      "outputs": [
+        {
+          "internalType": "uint256",
+          "name": "",
+          "type": "uint256"
+        }
+      ],
+      "stateMutability": "view",
+      "type": "function"
+    }
+  ],
+  "bin": "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",
+  "bin-runtime": "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",
+  "bin-pvm": "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"
+}
\ No newline at end of file
diff --git a/crates/anvil-polkadot/test-data/ImmutableStorage.sol b/crates/anvil-polkadot/test-data/ImmutableStorage.sol
new file mode 100644
index 0000000000000..32fa5a407ed70
--- /dev/null
+++ b/crates/anvil-polkadot/test-data/ImmutableStorage.sol
@@ -0,0 +1,19 @@
+pragma solidity ^0.8.0;
+
+contract ImmutableStorage {
+    uint256 public immutable immutableValue;
+    address public immutable immutableAddress;
+
+    constructor(uint256 _value, address _addr) {
+        immutableValue = _value;
+        immutableAddress = _addr;
+    }
+
+    function getImmutableValue() public view returns (uint256) {
+        return immutableValue;
+    }
+
+    function getImmutableAddress() public view returns (address) {
+        return immutableAddress;
+    }
+}
diff --git a/crates/anvil-polkadot/tests/it/abi.rs b/crates/anvil-polkadot/tests/it/abi.rs
index 0c0aedfc188ca..67489be13856d 100644
--- a/crates/anvil-polkadot/tests/it/abi.rs
+++ b/crates/anvil-polkadot/tests/it/abi.rs
@@ -23,3 +23,9 @@ sol!(
     SimpleStorageCaller,
     "test-data/SimpleStorageCaller.json"
 );
+
+sol!(
+    #[derive(Debug)]
+    ImmutableStorage,
+    "test-data/ImmutableStorage.json"
+);
diff --git a/crates/anvil-polkadot/tests/it/state_injector.rs b/crates/anvil-polkadot/tests/it/state_injector.rs
index c41522c23dc69..27a2f96b26e27 100644
--- a/crates/anvil-polkadot/tests/it/state_injector.rs
+++ b/crates/anvil-polkadot/tests/it/state_injector.rs
@@ -1,6 +1,6 @@
 use crate::{
-    abi::SimpleStorage,
-    utils::{ContractCode, TestNode, get_contract_code, unwrap_response},
+    abi::{ImmutableStorage, SimpleStorage},
+    utils::{ContractCode, TestNode, get_contract_code, get_contract_pvm_code, unwrap_response},
 };
 use alloy_eips::BlockId;
 use alloy_primitives::{Address, B256, Bytes, U256};
@@ -776,3 +776,116 @@ async fn test_set_storage() {
         assert_eq!(stored_value, 511);
     }
 }
+
+#[tokio::test(flavor = "multi_thread")]
+async fn test_set_immutable_storage() {
+    use alloy_sol_types::SolValue;
+
+    let anvil_node_config = AnvilNodeConfig::test_config();
+    let substrate_node_config = SubstrateNodeConfig::new(&anvil_node_config);
+    let mut node = TestNode::new(anvil_node_config, substrate_node_config).await.unwrap();
+
+    let alith = Account::from(subxt_signer::eth::dev::alith());
+    let alith_addr = Address::from(ReviveAddress::new(alith.address()));
+    let initial_value = U256::from(12345);
+    let initial_address = Address::from(ReviveAddress::new(
+        Account::from(subxt_signer::eth::dev::baltathar()).address(),
+    ));
+
+    // Deploy PVM contract with ABI-encoded constructor args
+    let bytecode = get_contract_pvm_code("ImmutableStorage");
+    let constructor_args = (initial_value, initial_address).abi_encode();
+    let deployment_bytecode = [bytecode.as_slice(), constructor_args.as_slice()].concat();
+    let tx_hash = node.deploy_contract(&deployment_bytecode, alith.address()).await;
+    unwrap_response::<()>(node.eth_rpc(EthRequest::Mine(None, None)).await.unwrap()).unwrap();
+
+    let contract_address = Address::from(ReviveAddress::new(
+        node.get_transaction_receipt(tx_hash).await.contract_address.unwrap(),
+    ));
+
+    // Verify initial immutable value
+    let call_tx = TransactionRequest::default()
+        .from(alith_addr)
+        .to(contract_address)
+        .input(TransactionInput::both(
+            ImmutableStorage::getImmutableValueCall {}.abi_encode().into(),
+        ));
+    let result = unwrap_response::<Bytes>(
+        node.eth_rpc(EthRequest::EthCall(call_tx.into(), None, None, None)).await.unwrap(),
+    )
+    .unwrap();
+    assert_eq!(
+        ImmutableStorage::getImmutableValueCall::abi_decode_returns(&result.0).unwrap(),
+        initial_value
+    );
+
+    // Verify initial immutable address
+    let call_tx = TransactionRequest::default()
+        .from(alith_addr)
+        .to(contract_address)
+        .input(TransactionInput::both(
+            ImmutableStorage::getImmutableAddressCall {}.abi_encode().into(),
+        ));
+    let result = unwrap_response::<Bytes>(
+        node.eth_rpc(EthRequest::EthCall(call_tx.into(), None, None, None)).await.unwrap(),
+    )
+    .unwrap();
+    assert_eq!(
+        ImmutableStorage::getImmutableAddressCall::abi_decode_returns(&result.0).unwrap(),
+        initial_address
+    );
+
+    // Set new immutable values via anvil_setImmutableStorageAt
+    // Each immutable is passed as a separate ABI-encoded bytes value
+    let new_value = U256::from(99999);
+    let new_address = Address::from(ReviveAddress::new(
+        Account::from(subxt_signer::eth::dev::charleth()).address(),
+    ));
+
+    let immutables = vec![
+        Bytes::from(new_value.abi_encode()),
+        Bytes::from(new_address.abi_encode()),
+    ];
+
+    unwrap_response::<()>(
+        node.eth_rpc(EthRequest::SetImmutableStorageAt(
+            contract_address,
+            immutables,
+        ))
+        .await
+        .unwrap(),
+    )
+    .unwrap();
+
+    // Verify new immutable value
+    let call_tx = TransactionRequest::default()
+        .from(alith_addr)
+        .to(contract_address)
+        .input(TransactionInput::both(
+            ImmutableStorage::getImmutableValueCall {}.abi_encode().into(),
+        ));
+    let result = unwrap_response::<Bytes>(
+        node.eth_rpc(EthRequest::EthCall(call_tx.into(), None, None, None)).await.unwrap(),
+    )
+    .unwrap();
+    assert_eq!(
+        ImmutableStorage::getImmutableValueCall::abi_decode_returns(&result.0).unwrap(),
+        new_value
+    );
+
+    // Verify new immutable address
+    let call_tx = TransactionRequest::default()
+        .from(alith_addr)
+        .to(contract_address)
+        .input(TransactionInput::both(
+            ImmutableStorage::getImmutableAddressCall {}.abi_encode().into(),
+        ));
+    let result = unwrap_response::<Bytes>(
+        node.eth_rpc(EthRequest::EthCall(call_tx.into(), None, None, None)).await.unwrap(),
+    )
+    .unwrap();
+    assert_eq!(
+        ImmutableStorage::getImmutableAddressCall::abi_decode_returns(&result.0).unwrap(),
+        new_address
+    );
+}
diff --git a/crates/anvil-polkadot/tests/it/utils.rs b/crates/anvil-polkadot/tests/it/utils.rs
index 0f220afebc4f7..a1eb4922180e9 100644
--- a/crates/anvil-polkadot/tests/it/utils.rs
+++ b/crates/anvil-polkadot/tests/it/utils.rs
@@ -461,22 +461,36 @@ pub struct ContractCode {
     pub runtime: Option<Vec<u8>>,
 }
 
-pub fn get_contract_code(name: &str) -> ContractCode {
+fn load_contract_json(name: &str) -> Value {
     let contract_path =
         std::path::PathBuf::from(env!("CARGO_MANIFEST_DIR")).join(format!("test-data/{name}.json"));
 
-    let contract_json: Value = serde_json::from_reader(std::io::BufReader::new(
+    serde_json::from_reader(std::io::BufReader::new(
         std::fs::File::open(contract_path).unwrap(),
     ))
-    .unwrap();
+    .unwrap()
+}
+
+fn decode_hex_field(json: &Value, field: &str) -> Option<Vec<u8>> {
+    json.get(field)
+        .and_then(|v| v.as_str())
+        .map(|s| hex::decode(s).unwrap())
+}
+
+pub fn get_contract_code(name: &str) -> ContractCode {
+    let contract_json = load_contract_json(name);
 
-    let init = hex::decode(contract_json.get("bin").unwrap().as_str().unwrap()).unwrap();
-    let runtime =
-        contract_json.get("bin-runtime").map(|code| hex::decode(code.as_str().unwrap()).unwrap());
+    let init = decode_hex_field(&contract_json, "bin").expect("missing 'bin' field");
+    let runtime = decode_hex_field(&contract_json, "bin-runtime");
 
     ContractCode { init, runtime }
 }
 
+pub fn get_contract_pvm_code(name: &str) -> Vec<u8> {
+    let contract_json = load_contract_json(name);
+    decode_hex_field(&contract_json, "bin-pvm").expect("missing 'bin-pvm' field")
+}
+
 /// Gets contract code with constructor arguments encoded and appended to bytecode.
 /// Loads the ABI from the contract JSON file and encodes the constructor arguments.
 pub fn get_contract_code_with_args(name: &str, constructor_args: Vec<DynSolValue>) -> ContractCode {
diff --git a/crates/anvil/core/src/eth/mod.rs b/crates/anvil/core/src/eth/mod.rs
index 1cfc62ba238a9..b717f665b152c 100644
--- a/crates/anvil/core/src/eth/mod.rs
+++ b/crates/anvil/core/src/eth/mod.rs
@@ -428,6 +428,14 @@ pub enum EthRequest {
         B256,
     ),
 
+    /// Sets the immutable data associated with a contract address
+    #[serde(rename = "anvil_setImmutableStorageAt")]
+    SetImmutableStorageAt(
+        Address,
+        /// immutable values (each as ABI-encoded bytes)
+        Vec<Bytes>,
+    ),
+
     /// Sets the coinbase address
     #[serde(rename = "anvil_setCoinbase", alias = "hardhat_setCoinbase", with = "sequence")]
     SetCoinbase(Address),
diff --git a/crates/anvil/src/eth/api.rs b/crates/anvil/src/eth/api.rs
index 06aa13dca1b02..b869601b5ff32 100644
--- a/crates/anvil/src/eth/api.rs
+++ b/crates/anvil/src/eth/api.rs
@@ -392,6 +392,11 @@ impl EthApi {
             EthRequest::SetStorageAt(addr, slot, val) => {
                 self.anvil_set_storage_at(addr, slot, val).await.to_rpc_result()
             }
+            EthRequest::SetImmutableStorageAt(_, _) => {
+                return ResponseResult::Error(RpcError::invalid_params(
+                    "anvil_setImmutableStorageAt is not supported on EVM anvil",
+                ));
+            }
             EthRequest::SetCoinbase(addr) => self.anvil_set_coinbase(addr).await.to_rpc_result(),
             EthRequest::EthCoinbase(()) => self.author().to_rpc_result(),
             EthRequest::SetChainId(id) => self.anvil_set_chain_id(id).await.to_rpc_result(),