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Release 2.6 (#279)
* Add EnhancedVolcano (#267) * add enh. volcano * update docu * add comment * rm comments, add omitNa * ch version to dev * add volcano to tests * fix deprecated key-value * update docs,changelog * prettier test rsem profile * fix contrast names * Apply color scheme of EnhancedVolcano to plotly volcano (#268) * add colors to plotly volcano * add color scale * Hotfix colors volcano plot (#271) * hotfix switch volcano colors * fix colors * add abs, rm space * rm "feature" text * update titles, legends * add subtitle for interactive plot * ch y-axis label * rm outcommented lines * Put gprofiler in tabs (#270) * put gprofiler in tabs * revert * linting * trim whitespace * mod plot title * Bugfixes pathway analysis (#273) * raise max dimensions limit * concat ensembl ids with gene names * update changelog & usage update changelog & usage update changelog & usage Co-authored-by: Famke Bäuerle <[email protected]> fix typo * Update report (#275) * change report name * modify report * update report & tests & fix volcano colors * add to changelog, references * fix citation * fix df RIN * fix linting & ci * fix padding * update md5sums due to sampleNames * apply suggestions * update confs * apply suggestion * add num all genes * change icon * adjust docs text * Incorporate Feedback and fix typos (#276) * incorporate feedback * update report * fix typos * update changelog * fix pre-commit * fix spaces * fix colored cells * linting * fix typos * adjust table border * fix typos * apply suggestions * apply suggestions * Bugfix summary (#278) * fix contrast * update usage * add to changelog * update test * PR num * ch md5sum * Add gene biotypes to interactive volcano plots (#272) * adjust md5sums due to gtf biotypes * update branch * fix assignment * add biotype to final DE table * update md5sums * version bump to 2.6 (#277) * update branch.yml * apply suggestion lint * add to ignore * update branch.yml * test docker download * rm pull_request_target rule * update branch protection * update branch protection * update branch protection * revert branch yml * update branch yml * test download * test update * update branch.yml * change publishdir mode * update version test * Bugfix volcano (#280) * fix thresholds in enhanced volcano * add to changelog * Prettify long contrast names in plot titles (#281) * prettify long contrast names in plot titles * add new test profile but not for ci * rename files * correct pr nums * apply suggestions (#282) * update env name * revert download.yml * test apptainer * change order * revert download.yml * revert cachedir * update md5sum * Modify input base (#283) * switch back to githubusercontent * add to changelog
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.github/workflows/branch.yml

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name: nf-core branch protection
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# This workflow is triggered on PRs to master branch on the repository
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# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev`
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# This workflow is triggered on PRs to `main`/`master` branch on the repository
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# It fails when someone tries to make a PR against the nf-core `main`/`master` branch instead of `dev`
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on:
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pull_request_target:
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branches: [master]
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branches:
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- main
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- master
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jobs:
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test:
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runs-on: ubuntu-latest
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steps:
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# PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
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# PRs to the nf-core repo main/master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
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- name: Check PRs
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if: github.repository == 'qbic-pipelines/rnadeseq'
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run: |
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{ [[ ${{github.event.pull_request.head.repo.full_name }} == qbic-pipelines/rnadeseq ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
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# If the above check failed, post a comment on the PR explaining the failure {%- raw %}
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# If the above check failed, post a comment on the PR explaining the failure
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# NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
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- name: Post PR comment
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if: failure()
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uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
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with:
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message: |
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## This PR is against the `master` branch :x:
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## This PR is against the `${{github.event.pull_request.base.ref}}` branch :x:
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* Do not close this PR
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* Click _Edit_ and change the `base` to `dev`
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Hi @${{ github.event.pull_request.user.login }},
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It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch.
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The `master` branch on nf-core repositories should always contain code from the latest release.
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Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
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It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) ${{github.event.pull_request.base.ref}} branch.
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The ${{github.event.pull_request.base.ref}} branch on nf-core repositories should always contain code from the latest release.
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Because of this, PRs to ${{github.event.pull_request.base.ref}} are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
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You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
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Note that even after this, the test will continue to show as failing until you push a new commit.
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Thanks again for your contribution!
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repo-token: ${{ secrets.GITHUB_TOKEN }}
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allow-repeats: false {%- endraw %}
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allow-repeats: false

.github/workflows/ci.yml

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environment.yml
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- name: Build new docker image
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if: env.MATCHED_FILES
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.5
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.6
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# Change the version above and the third version below before/after release
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- name: Pull docker image
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if: ${{ !env.MATCHED_FILES }}
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run: |
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docker pull ghcr.io/qbic-pipelines/rnadeseq:dev
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.5
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.6
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- name: Install Nextflow
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uses: nf-core/setup-nextflow@v1
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environment.yml
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- name: Build new docker image
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if: env.MATCHED_FILES
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.5
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.6
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# Change the version above and the third version below before/after release
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- name: Pull docker image
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if: ${{ !env.MATCHED_FILES }}
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run: |
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docker pull ghcr.io/qbic-pipelines/rnadeseq:dev
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.5
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.6
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- name: Install Nextflow
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uses: nf-core/setup-nextflow@v1
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name: Test successful pipeline download with 'nf-core download'
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name: Test successful pipeline download with 'nf-core pipelines download'
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# Run the workflow when:
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# - dispatched manually
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# - when a PR is opened or reopened to master branch
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# - when a PR is opened or reopened to main/master branch
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# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev.
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on:
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workflow_dispatch:
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inputs:
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testbranch:
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description: "The specific branch you wish to utilize for the test execution of nf-core download."
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description: "The specific branch you wish to utilize for the test execution of nf-core pipelines download."
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required: true
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default: "dev"
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pull_request:
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types:
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- opened
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- edited
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- synchronize
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branches:
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- master
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pull_request_target:
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branches:
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- main
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- master
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env:
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NXF_ANSI_LOG: false
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jobs:
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configure:
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runs-on: ubuntu-latest
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outputs:
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REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }}
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REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }}
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REPO_BRANCH: ${{ steps.get_repo_properties.outputs.REPO_BRANCH }}
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steps:
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- name: Get the repository name and current branch
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id: get_repo_properties
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run: |
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echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT"
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echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT"
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echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT"
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download:
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needs: configure
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- name: Install Nextflow
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uses: nf-core/setup-nextflow@v2
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- name: Disk space cleanup
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uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
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- uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
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- uses: actions/setup-python@a26af69be951a213d495a4c3e4e4022e16d87065 # v5
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with:
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python-version: "3.12"
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python-version: "3.13"
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architecture: "x64"
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- uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
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- name: Setup Apptainer
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uses: eWaterCycle/setup-apptainer@4bb22c52d4f63406c49e94c804632975787312b3 # v2.0.0
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with:
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singularity-version: 3.8.3
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apptainer-version: 1.3.4
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- name: Install dependencies
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python -m pip install --upgrade pip
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pip install git+https://github.com/nf-core/tools.git@dev
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- name: Get the repository name and current branch set as environment variable
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- name: Make a cache directory for the container images
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run: |
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echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV}
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echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
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echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV}
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mkdir -p ./singularity_container_images
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- name: Download the pipeline
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env:
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NXF_SINGULARITY_CACHEDIR: ./
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NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
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run: |
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nf-core download ${{ env.REPO_LOWERCASE }} \
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--revision ${{ env.REPO_BRANCH }} \
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--outdir ./${{ env.REPOTITLE_LOWERCASE }} \
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nf-core pipelines download ${{ needs.configure.outputs.REPO_LOWERCASE }} \
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--revision ${{ needs.configure.outputs.REPO_BRANCH }} \
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--outdir ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} \
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--compress "none" \
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--container-system 'singularity' \
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--container-library "quay.io" -l "docker.io" -l "ghcr.io" \
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--container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io/library/" -l "ghcr.io" \
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--container-cache-utilisation 'amend' \
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--download-configuration
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--download-configuration 'yes'
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- name: Inspect download
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run: tree ./${{ env.REPOTITLE_LOWERCASE }}
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run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}
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- name: Inspect container images
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run: tree ./singularity_container_images | tee ./container_initial
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- name: Count the downloaded number of container images
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id: count_initial
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run: |
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image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
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echo "Initial container image count: $image_count"
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echo "IMAGE_COUNT_INITIAL=$image_count" >> "$GITHUB_OUTPUT"
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- name: Count the downloaded number of container images
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id: count_afterwards
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run: |
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image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
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echo "Post-pipeline run container image count: $image_count"
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echo "IMAGE_COUNT_AFTER=$image_count" >> "$GITHUB_OUTPUT"
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- name: Compare container image counts
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id: count_comparison
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run: |
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if [ "${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}" -ne "${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}" ]; then
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initial_count=${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}
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final_count=${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}
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difference=$((final_count - initial_count))
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echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!"
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tree ./singularity_container_images > ./container_afterwards
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diff ./container_initial ./container_afterwards
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exit 1
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else
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echo "The pipeline can be downloaded successfully!"
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fi
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- name: Run the downloaded pipeline (stub)
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id: stub_run_pipeline
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continue-on-error: true
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env:
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NXF_SINGULARITY_CACHEDIR: ./
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NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
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NXF_SINGULARITY_HOME_MOUNT: true
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run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
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run: nextflow run ./${{needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
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- name: Run the downloaded pipeline (stub run not supported)
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id: run_pipeline
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if: ${{ job.steps.stub_run_pipeline.status == failure() }}
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if: ${{ steps.stub_run_pipeline.outcome == 'failure' }}
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env:
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NXF_SINGULARITY_CACHEDIR: ./
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NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
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NXF_SINGULARITY_HOME_MOUNT: true
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run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
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run: nextflow run ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -profile test,singularity --outdir ./results
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- name: Upload Nextflow logfile for debugging purposes
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uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
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with:
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name: nextflow_logfile.txt
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path: .nextflow.log*
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include-hidden-files: true

.github/workflows/linting.yml

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python-version: "3.12"
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architecture: "x64"
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- name: read .nf-core.yml
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uses: pietrobolcato/action-read-yaml@9f13718d61111b69f30ab4ac683e67a56d254e1d # 1.1.0
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id: read_yml
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with:
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config: ${{ github.workspace }}/.nf-core.yml
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- name: Install dependencies
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run: |
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python -m pip install --upgrade pip
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pip install nf-core
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pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
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env:

.github/workflows/push_github_container_reg.yml

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- name: Push image to GHCR (dev)
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if: ${{ github.event_name == 'push' }}
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run: |
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docker tag ghcr.io/qbic-pipelines/rnadeseq:latest ghcr.io/qbic-pipelines/rnadeseq:dev
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docker push ghcr.io/qbic-pipelines/rnadeseq:dev
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docker tag ghcr.io/qbic-pipelines/rnadeseq:latest ghcr.io/qbic-pipelines/rnadeseq:2.6
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docker push ghcr.io/qbic-pipelines/rnadeseq:2.6
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if: ${{ github.event_name == 'release' }}

.gitignore

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.nextflow*
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work/
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data/
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results/
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results*/
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.DS_Store
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testing/
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testing*

.nf-core.yml

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- .github/ISSUE_TEMPLATE/bug_report.yml
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- .github/PULL_REQUEST_TEMPLATE.md
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- .github/workflows/branch.yml
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- .github/workflows/linting.yml
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- .gitignore
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- assets/email_template.txt
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- assets/sendmail_template.txt

CHANGELOG.md

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The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
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and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
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## 2.6 - Tree Roots
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### Added
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- [#267](https://github.com/qbic-pipelines/rnadeseq/pull/267) Add EnhancedVolcano plots and export to output results
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- [#275](https://github.com/qbic-pipelines/rnadeseq/pull/275) Added workflow section to report with mermaid graph
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- [#272](https://github.com/qbic-pipelines/rnadeseq/pull/272) Add gene biotypes from gtf to DE_tables and volcano plots
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### Changed
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- [#267](https://github.com/qbic-pipelines/rnadeseq/pull/267) Export static and interactive plotly volcano plots
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- [#268](https://github.com/qbic-pipelines/rnadeseq/pull/268) Apply EnhancedVolcano color code to plotly volcano plots
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- [#269](https://github.com/qbic-pipelines/rnadeseq/pull/269) Put gprofiler manhattan plots in tabs
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- [#275](https://github.com/qbic-pipelines/rnadeseq/pull/275) Changed report headers, citations, links
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- [#276](https://github.com/qbic-pipelines/rnadeseq/pull/276) Adjusted text, fixed typos, `--path_quote` is deprecated
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- [#277](https://github.com/qbic-pipelines/rnadeseq/pull/277) Version bump to 2.6
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- [#281](https://github.com/qbic-pipelines/rnadeseq/pull/281) Prettify long contrast names in volcano plot titles
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- [#282](https://github.com/qbic-pipelines/rnadeseq/pull/282) Apply text suggestion changes from review [#279](https://github.com/qbic-pipelines/rnadeseq/pull/279)
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### Fixed
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- [#271](https://github.com/qbic-pipelines/rnadeseq/pull/271) Fix colors in plotly volcano plots
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- [#273](https://github.com/qbic-pipelines/rnadeseq/pull/272) Fix duplicate row.names in pathway analysis and max dimensions error
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- [#274](https://github.com/qbic-pipelines/rnadeseq/pull/274) Fix colors volcano plotly, sample dist. heatmap sampleNames, header trim whitespaces
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- [#278](https://github.com/qbic-pipelines/rnadeseq/pull/278) Fix summary contrast names
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- [#280](https://github.com/qbic-pipelines/rnadeseq/pull/280) Fix thresholds and lines in volcano plots
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- [#283](https://github.com/qbic-pipelines/rnadeseq/pull/283) Fix test config inputs
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## 2.5 - The Potato Eaters
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### Added
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### Changed
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- [#115](https://github.com/qbic-pipelines/rnadeseq/pull/115) Template update
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- [#117](https://github.com/qbic-pipelines/rnadeseq/pull/117) Turned LabID optional for report output in RNAseq_report.Rmd
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- [#117](https://github.com/qbic-pipelines/rnadeseq/pull/117) Turned LabID optional for report output in rnadeseq_report.Rmd
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- Removed assets/report_options.yml
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- [#110](https://github.com/qbic-pipelines/rnadeseq/pull/110) Changed report to use rlog normalization by default, vst is used if --skip_rlog is enabled
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Dockerfile

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@@ -5,14 +5,14 @@ LABEL org.opencontainers.image.authors="Gisela Gabernet, Alexander Peltzer, Oska
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LABEL org.opencontainers.image.licenses=MIT
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COPY environment.yml /
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#RUN conda install -c conda-forge mamba
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RUN mamba env create --file /environment.yml -p /opt/conda/envs/qbic-pipelines-rnadeseq-2.5 && \
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RUN mamba env create --file /environment.yml -p /opt/conda/envs/qbic-pipelines-rnadeseq-2.6 && \
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mamba clean --all --yes
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RUN apt-get update -qq && \
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apt-get install -y zip procps ghostscript
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ENV PATH /opt/conda/envs/qbic-pipelines-rnadeseq-2.5/bin:$PATH
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ENV PATH=/opt/conda/envs/qbic-pipelines-rnadeseq-2.6/bin:$PATH
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# Dump the details of the installed packates to a file for posterity
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RUN mamba env export --name qbic-pipelines-rnadeseq-2.6 > qbic-pipelines-rnadeseq-2.6.yml
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# Instruct R processes to use these empty files instead of clashing with a local config
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assets/corp-styles.css

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}
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h1 {
6-
color: rgb(3, 101, 192);
6+
color: #0365c0;
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font-size: 127%;
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}
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@@ -17,6 +17,7 @@ h2 {
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}
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h3 {
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color: rgb(3, 101, 192);
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font-size: 109%;
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font-weight: bold;
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}

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