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Merge pull request #248 from WackerO/versions_2_4
4 - Bumped versions to 2.4 for next release
2 parents df4c728 + 5d143bb commit 73a712a

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.github/workflows/ci.yml

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environment.yml
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- name: Build new docker image
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if: env.MATCHED_FILES
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.3
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.4
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# Change the version above and the third version below before/after release
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- name: Pull docker image
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if: ${{ !env.MATCHED_FILES }}
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run: |
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docker pull ghcr.io/qbic-pipelines/rnadeseq:dev
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.3
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.4
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- name: Install Nextflow
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uses: nf-core/setup-nextflow@v1
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environment.yml
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- name: Build new docker image
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if: env.MATCHED_FILES
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.3
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run: docker build --no-cache . -t ghcr.io/qbic-pipelines/rnadeseq:2.4
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# Change the version above and the third version below before/after release
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- name: Pull docker image
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if: ${{ !env.MATCHED_FILES }}
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run: |
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docker pull ghcr.io/qbic-pipelines/rnadeseq:dev
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.3
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docker tag ghcr.io/qbic-pipelines/rnadeseq:dev ghcr.io/qbic-pipelines/rnadeseq:2.4
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- name: Install Nextflow
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uses: nf-core/setup-nextflow@v1

CHANGELOG.md

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The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
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and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
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## dev
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## 2.4 - A Pair of Shoes
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### Added
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- [#245](https://github.com/qbic-pipelines/rnadeseq/pull/245) Added background gene list to pathway analysis output
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### Changed
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- [#249](https://github.com/qbic-pipelines/rnadeseq/pull/249) Release 2.4
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- [#248](https://github.com/qbic-pipelines/rnadeseq/pull/248) Version bumps for release 2.4
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- [#247](https://github.com/qbic-pipelines/rnadeseq/pull/247) Changed the report text that shows when the gprofiler GMT file could not be downloaded
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### Removed

Dockerfile

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@@ -5,14 +5,14 @@ LABEL org.opencontainers.image.authors="Gisela Gabernet, Alexander Peltzer, Oska
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LABEL org.opencontainers.image.licenses=MIT
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COPY environment.yml /
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#RUN conda install -c conda-forge mamba
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RUN mamba env create --file /environment.yml -p /opt/conda/envs/qbic-pipelines-rnadeseq-dev && \
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RUN mamba env create --file /environment.yml -p /opt/conda/envs/qbic-pipelines-rnadeseq-2.4 && \
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mamba clean --all --yes
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RUN apt-get update -qq && \
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apt-get install -y zip procps ghostscript
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# Add conda installation dir to PATH
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ENV PATH /opt/conda/envs/qbic-pipelines-rnadeseq-dev/bin:$PATH
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ENV PATH /opt/conda/envs/qbic-pipelines-rnadeseq-2.4/bin:$PATH
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# Dump the details of the installed packates to a file for posterity
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RUN mamba env export --name qbic-pipelines-rnadeseq-dev > qbic-pipelines-rnadeseq-dev.yml
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RUN mamba env export --name qbic-pipelines-rnadeseq-2.4 > qbic-pipelines-rnadeseq-2.4.yml
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# Instruct R processes to use these empty files instead of clashing with a local config
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RUN touch .Rprofile
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RUN touch .Renviron

docs/usage.md

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The typical command for running the pipeline is as follows:
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```bash
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nextflow run qbic-pipelines/rnadeseq -r 2.3 -profile docker \
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nextflow run qbic-pipelines/rnadeseq -r 2.4 -profile docker \
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--gene_counts 'merged_gene_counts.txt' \
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--input 'QXXXX_sample_preparations.tsv' \
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--model 'linear_model.txt' \
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Afterwards, you should be able to also run test_star_rsem and test_star_salmon from qbic-pipelines/rnadeseq without manually cloning, e.g.:
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```bash
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nextflow run qbic-pipelines/rnadeseq -r 2.3 -profile docker,test_star_salmon
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nextflow run qbic-pipelines/rnadeseq -r 2.4 -profile docker,test_star_salmon
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```
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### Reproducibility

environment.yml

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# You can use this file to create a conda environment for this pipeline:
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# conda env create -f environment.yml
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# use this to find packages: https://anaconda.org/
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name: qbic-pipelines-rnadeseq-dev
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name: qbic-pipelines-rnadeseq-2.4
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channels:
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- bioconda
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- conda-forge

modules/local/report.nf

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process REPORT {
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container 'ghcr.io/qbic-pipelines/rnadeseq:dev'
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container 'ghcr.io/qbic-pipelines/rnadeseq:2.4'
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input:
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path gene_counts

nextflow.config

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description = """Differential gene expression analysis and pathway analysis of RNAseq data"""
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mainScript = 'main.nf'
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nextflowVersion = '!>=23.04.0'
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version = 'dev'
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version = '2.4'
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doi = ''
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}
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