Hi, Could you please look what is the reason for such segmented plots? 60x coverage <img width="1507" alt="Image" src="https://github.com/user-attachments/assets/1a71c10c-2f3b-4c62-95a0-7719e1e79120" /> 20x coverage <img width="1498" alt="Image" src="https://github.com/user-attachments/assets/ac4e6fb7-012e-4ddc-9cd6-5d8deee8cad7" /> While 120x gives complete segments: <img width="1519" alt="Image" src="https://github.com/user-attachments/assets/07ae22f0-fcac-4c84-a8ea-aec75df426ef" />