diff --git a/tests/functional/object/rgw/test_rgw_read_affinity.py b/tests/functional/object/rgw/test_rgw_read_affinity.py
new file mode 100644
index 00000000000..c87ee1e94b8
--- /dev/null
+++ b/tests/functional/object/rgw/test_rgw_read_affinity.py
@@ -0,0 +1,133 @@
+import logging
+import pytest
+
+from ocs_ci.framework import config
+from ocs_ci.framework.pytest_customization.marks import (
+    red_squad,
+    tier1,
+    rgw,
+    skipif_no_lso,
+)
+from ocs_ci.ocs.resources.pod import get_rgw_pods
+from ocs_ci.ocs.ocp import OCP
+import ocs_ci.ocs.resources.pod as pod
+from ocs_ci.ocs import constants
+from ocs_ci.utility.utils import TimeoutSampler, run_cmd
+
+log = logging.getLogger(__name__)
+
+CEPH_OBJECT_STORE = OCP(
+    kind="CephObjectStore", namespace=config.ENV_DATA["cluster_namespace"]
+)
+OCP_OBJ = OCP(kind=constants.POD, namespace=config.ENV_DATA["cluster_namespace"])
+DEFAULT_READ_AFFINITY = "localize"
+
+
+@rgw
+@red_squad
+@skipif_no_lso
+class TestRGWReadAffinityMode:
+    """
+    Test the RGW Read Affinity value in an ODF cluster
+
+    """
+
+    @pytest.fixture(scope="class", autouse=True)
+    def revert_read_affinity_mode(self, request):
+        """
+        Reverts the RGW readAffinity mode to localize state.
+        """
+
+        def teardown():
+            patch = '\'{"spec": {"gateway": {"readAffinity": {"type": "localize"}}}}\''
+            patch_cmd = (
+                f"oc patch cephObjectStore/{CEPH_OBJECT_STORE.data['items'][0]['metadata'].get('name')} "
+                f"-n openshift-storage  --type merge --patch {patch}"
+            )
+            run_cmd(patch_cmd)
+            sample = TimeoutSampler(
+                timeout=60,
+                sleep=10,
+                func=self.get_read_affinity_from_ceph,
+            )
+            sample.wait_for_func_value(DEFAULT_READ_AFFINITY)
+
+        request.addfinalizer(teardown)
+
+    def get_read_affinity_from_ceph(self):
+        """
+        Returns current readAffinity set in ceph cluster
+        Returns :
+            ceph_read_affinity_mode (String)
+        """
+        cmd = "ceph config show client.rgw.ocs.storagecluster.cephobjectstore.a"
+        ceph_pod = pod.get_ceph_tools_pod()
+        ceph_op = ceph_pod.exec_ceph_cmd(cmd)
+        for val in ceph_op:
+            if val["name"] == "rados_replica_read_policy":
+                log.info(val["value"])
+                return val["value"]
+        else:
+            return None
+
+    @tier1
+    def test_rgw_read_affinity_mode(self):
+        """
+        Test default ReadAffinity mode of RGW in an ODF cluster
+            step #1. Validate readAffinity mode is set to ""local"" for RGW
+            step #2. Validate the status of RGW pod
+            step #3. Validate readAffinity value from ceph cluster
+            step #4: Change current mode to "balanced" and validate it along with RGW pod status
+            step #5: Change current mode to "default" and validate it along with RGW pod status
+        """
+        rgw_pod_count = len(get_rgw_pods())
+        # 1. Validate readAffinity mode is set to "localize" for RGW
+        current_read_affinity = CEPH_OBJECT_STORE.data["items"][0]["spec"]["gateway"][
+            "readAffinity"
+        ]["type"]
+        assert (
+            DEFAULT_READ_AFFINITY == current_read_affinity
+        ), f"Default ReadAffinity for RGW is {current_read_affinity}. Expected value is {DEFAULT_READ_AFFINITY}"
+        # 2. Validate the status of RGW pod
+        assert OCP_OBJ.wait_for_resource(
+            condition=constants.STATUS_RUNNING,
+            resource_count=rgw_pod_count,
+            selector=constants.RGW_APP_LABEL,
+        )
+        # 3. Validate readAffinity value from ceph cluster
+        sample = TimeoutSampler(
+            timeout=60,
+            sleep=10,
+            func=self.get_read_affinity_from_ceph,
+        )
+        sample.wait_for_func_value(DEFAULT_READ_AFFINITY)
+
+        # 4 and 5. Change current mode to all available mode and validate it along with RGW pod status
+        READ_AFFINITY_LIST = ["balance", "default"]
+        for val in READ_AFFINITY_LIST:
+            patch = f'\'{{"spec": {{"gateway": {{"readAffinity": {{"type": "{val}"}}}}}}}}\''
+            patch_cmd = (
+                f"oc patch cephObjectStore/{CEPH_OBJECT_STORE.data['items'][0]['metadata'].get('name')} "
+                f"-n openshift-storage  --type merge --patch {patch}"
+            )
+            run_cmd(patch_cmd)
+            CEPH_OBJECT_STORE.reload_data()
+            current_read_affinity = CEPH_OBJECT_STORE.data["items"][0]["spec"][
+                "gateway"
+            ]["readAffinity"]["type"]
+            assert (
+                current_read_affinity == val
+            ), f"Failed to change ReadAffinity for RGW. Current value: {current_read_affinity}. Expected value: {val}"
+
+            # Validate RGW POD status
+            assert OCP_OBJ.wait_for_resource(
+                condition=constants.STATUS_RUNNING,
+                resource_count=rgw_pod_count,
+                selector=constants.RGW_APP_LABEL,
+            )
+            sample = TimeoutSampler(
+                timeout=60,
+                sleep=10,
+                func=self.get_read_affinity_from_ceph,
+            )
+            sample.wait_for_func_value(val)