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Vclust is an alignment-based tool for fast and accurate calculation of Average Nucleotide Identity (ANI) between complete or metagenomically-assembled viral genomes. The tool also performs ANI-based clustering of genomes according to standards recommended by international virus consortia, including *International Committee on Taxonomy of Viruses* (ICTV) and *Minimum Information about an Uncultivated Virus Genome* (MIUViG).
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