running with a merged fastq file

[yeroslaviz]

Thank you @rrwick for a great tool.

I was wondering what the reason behind the --kmers parameter.

I have samples which were sequenced twice, the second time was done to increase the sequencing depth. Unfortunately the second run was done with a different machine and a different read length.

When I try to run unicycler on the merged fastq files i it fails when it tries to calculate kmers of length 61. the older fastq file is only 60nt long.

Does it make more sense to give the too a specific list of parameters e.g. --kmers 13,25,33,39,45,49,53,57 or is it better to work with the files separately

thanks