@@ -20,29 +20,47 @@ We welcome suggestions, ideas, and contributions! Please do not hesitate to cont
2020A set of extensive vignettes can be found in the [ LIANA+ documentation] ( https://liana-py.readthedocs.io/en/latest/ ) .
2121
2222## Decision Tree
23- ### Does the data contain spatial coordinates?
24- #### Yes
25- - ** Q: Bivariate or unsupervised, multi-variate, and multi-view analysis?**
26- - ** Bivariate:**
27- - ** Q: Are you interested in identifying the subregions of interactions (i.e., local interactions)?**
28- - ** Yes:** Check the [ ** Local** Bivariate Metrics] ( https://liana-py.readthedocs.io/en/latest/notebooks/bivariate.html#Bivariate-Ligand-Receptor-Relationships )
29- - ** No:** Check the [ ** Global** Bivariate Metrics] ( https://liana-py.readthedocs.io/en/latest/notebooks/bivariate.html#Bivariate-Ligand-Receptor-Relationships )
30- - ** Unsupervised:** [ Multi-view learning] ( https://liana-py.readthedocs.io/en/latest/notebooks/misty.html )
31-
32- #### No
33- - ** Q: Are you interested in comparing CCC across samples?**
34- - ** Yes:**
35- - ** Q: Are you interested in a specific contrast?**
36- - ** Yes:** [ Differential Contrasts and Downstream Signalling] ( https://liana-py.readthedocs.io/en/latest/notebooks/targeted.html )
37- - ** No:** Unsupervised Cross-conditional LR inference with [ MOFA+] ( https://liana-py.readthedocs.io/en/latest/notebooks/mofatalk.html ) or [ Tensor-cell2cell] ( https://liana-py.readthedocs.io/en/latest/notebooks/liana_c2c.html )
38- - ** No:** [ Steady-state Ligand-Receptor inference] ( https://liana-py.readthedocs.io/en/latest/notebooks/basic_usage.html )
39-
40- ### Is your data Multi-modal?
41- - ** Spatial:** [ Integrating Multi-Modal Spatially-Resolved Technologies] ( https://liana-py.readthedocs.io/en/latest/notebooks/sma.html )
42- - ** Non-Spatial:** [ Integrating Multi-Modal Single-Cell Technologies] ( https://liana-py.readthedocs.io/en/latest/notebooks/sc_multi.html )
43-
44- #### Infer Metabolite-mediated CCC from transcriptomics?
45- - [ Non-spatial Data] ( https://liana-py.readthedocs.io/en/latest/notebooks/sc_multi.html#Metabolite-mediated-CCC-from-Transcriptomics-Data )
23+
24+ ``` mermaid
25+ flowchart TD
26+ Start[What type of data?] --> Spatial{Spatial<br/>coordinates?}
27+ Start --> Modal{Multi-modal?}
28+
29+ %% Spatial branch
30+ Spatial -->|Yes| SpatialRes{Resolution?}
31+ SpatialRes -->|Single-cell| Inflow[Inflow Score]
32+ SpatialRes -->|Spot-based| SpatialType{Analysis type?}
33+ SpatialType -->|Bivariate| LocalQ{Local<br/>interactions?}
34+ LocalQ -->|Yes| Local[Local Bivariate Metrics]
35+ LocalQ -->|No| Global[Global Bivariate Metrics]
36+ SpatialType -->|Unsupervised| MISTy[Multi-view Learning]
37+
38+ %% Non-spatial branch
39+ Spatial -->|No| Compare{Compare across<br/>samples?}
40+ Compare -->|Yes| Contrast{Specific<br/>contrast?}
41+ Contrast -->|Yes| Targeted[Differential Contrasts]
42+ Contrast -->|No| Unsup[MOFA+ / Tensor-cell2cell]
43+ Compare -->|No| Steady[Steady-state LR Inference]
44+
45+ %% Multi-modal branch
46+ Modal -->|Spatial| SMA[Multi-Modal Spatial]
47+ Modal -->|Non-Spatial| SCMulti[Multi-Modal Single-Cell]
48+
49+ %% Metabolite sub-branch
50+ SCMulti --> Metab[Metabolite-mediated CCC]
51+
52+ %% Links (click events)
53+ click Inflow "https://liana-py.readthedocs.io/en/latest/notebooks/inflow_score.html"
54+ click Local "https://liana-py.readthedocs.io/en/latest/notebooks/bivariate.html"
55+ click Global "https://liana-py.readthedocs.io/en/latest/notebooks/bivariate.html"
56+ click MISTy "https://liana-py.readthedocs.io/en/latest/notebooks/misty.html"
57+ click Targeted "https://liana-py.readthedocs.io/en/latest/notebooks/targeted.html"
58+ click Unsup "https://liana-py.readthedocs.io/en/latest/notebooks/mofatalk.html"
59+ click Steady "https://liana-py.readthedocs.io/en/latest/notebooks/basic_usage.html"
60+ click SMA "https://liana-py.readthedocs.io/en/latest/notebooks/sma.html"
61+ click SCMulti "https://liana-py.readthedocs.io/en/latest/notebooks/sc_multi.html"
62+ click Metab "https://liana-py.readthedocs.io/en/latest/notebooks/sc_multi.html#Metabolite-mediated-CCC"
63+ ```
4664
4765## API
4866For further information please check LIANA's [ API documentation] ( https://liana-py.readthedocs.io/en/latest/api.html ) .
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