forked from nf-core/strict-syntax-health
-
Notifications
You must be signed in to change notification settings - Fork 0
Expand file tree
/
Copy pathreadmapping_help.txt
More file actions
81 lines (62 loc) · 4.34 KB
/
readmapping_help.txt
File metadata and controls
81 lines (62 loc) · 4.34 KB
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
$ NXF_SYNTAX_PARSER=v2 nextflow run . --help
N E X T F L O W ~ version 26.03.1-edge
Launching `./main.nf` [adoring_woese] revision: 861ae59bc6
WARN: Unrecognized config option 'timeline.fields'
WARN: Unrecognized config option 'validation.defaultIgnoreParams'
WARN: Unrecognized config option 'validation.monochromeLogs'
------------------------------------------------------
_____ _______ _
/ ____| |__ __| | |
| (___ __ _ _ __ __ _ ___ _ __ ___ | | ___ | |
\___ \ / _` | '_ \ / _` |/ _ \ '__||___|| |/ _ \| |
____) | (_| | | | | (_| | __/ | | | (_) | |____
|_____/ \__,_|_| |_|\__, |\___|_| |_|\___/|______|
__/ |
|___/
sanger-tol/readmapping 1.4.0dev
------------------------------------------------------
Typical pipeline command:
nextflow run sanger-tol/readmapping -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>
help message of that parameter will be printed.
or `--helpFull`.
Input/output options
--input [string] Path to comma-separated file containing information about the samples in the experiment.
--outdir [string] The output directory where the results will be saved. You have to use absolute paths to storage on
Cloud infrastructure.
--outfmt [string] Format for output alignment files. Accepts options: "bam" or "cram". [default: cram]
--compression [string] Output file compression type. Currently accepts: "none" or "crumble" [default: crumble]
--merge_output [string] Set to a non-empty string to merge output alignment files by specimens. The string is used to name
the output directory
--coverage_binsize [integer] Bin size (in bp) used for computing coverage depth in the output bedGraph file. [default: 1000]
--pacbio_adapter_fasta [string] Path to FASTA file contains adapter sequences for PacBio HiFi reads. [default:
${projectDir}/assets/HiFi_adapter.fasta]
--pacbio_adapter_yaml [string] Path to YAML file containing guide for trimming/filtering PacBio data. See hifi-trimmer docs for
details: https://github.com/sanger-tol/hifi-trimmer [default:
${projectDir}/assets/HiFi_adapter.yaml]
--pacbio_uli_adapter [string] Path to FASTA file containing the PacBio ULI adapter sequence.
--email [string] Email address for completion summary.
Reference genome options
--fasta [string] Path to FASTA genome file.
--bwamem2_index [string] Path to directory or tar.gz archive for pre-built BWAMEM2 index.
--header [string] Optional template header file for BAM/CRAM outputs
Aligner options
--short_aligner [string] Alignment method to use for short-reads (options are: "minimap2" or "bwamem2" (accepted:
minimap2, bwamem2) [default: bwamem2]
--long_reads_map_chunk_size [integer] Chunk size for parallel alignment of long read data, in number of sequences [default: 10000]
--short_reads_map_chunk_size [integer] Chunk size for parallel alignment of short read data, in number of cram containers [default:
10000]
Generic options
--multiqc_title [string] MultiQC report title. Printed as page header, used for filename if not otherwise specified.
--multiqc_logo [string] Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file
--multiqc_methods_description [string] Custom MultiQC yaml file containing HTML including a methods description.
--help [boolean, string] Display the help message.
--help_full [boolean] Display the full detailed help message.
--show_hidden [boolean] Display hidden parameters in the help message (only works when --help or --help_full are provided).
!! Hiding 16 param(s), use the `--showHidden` parameter to show them !!
------------------------------------------------------
* The pipeline
https://doi.org/10.5281/zenodo.6563577
* The nf-core framework
https://doi.org/10.1038/s41587-020-0439-x
* Software dependencies
https://github.com/sanger-tol/readmapping/blob/main/CITATIONS.md