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variantcalling_help.txt
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$ NXF_SYNTAX_PARSER=v2 nextflow run . --help
N E X T F L O W ~ version 26.03.0-edge
Launching `./main.nf` [adoring_planck] revision: 0e3e4d38c9
WARN: Unrecognized config option 'validation.defaultIgnoreParams'
WARN: Unrecognized config option 'validation.monochromeLogs'
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_____ _______ _
/ ____| |__ __| | |
| (___ __ _ _ __ __ _ ___ _ __ ___ | | ___ | |
\___ \ / _` | '_ \ / _` |/ _ \ '__||___|| |/ _ \| |
____) | (_| | | | | (_| | __/ | | | (_) | |____
|_____/ \__,_|_| |_|\__, |\___|_| |_|\___/|______|
__/ |
|___/
sanger-tol/variantcalling 1.2.0dev
------------------------------------------------------
Typical pipeline command:
nextflow run sanger-tol/variantcalling -profile <docker/singularity/.../institute> --input samplesheet.csv --fasta genome.fasta.gz --outdir <OUTDIR>
help message of that parameter will be printed.
or `--helpFull`.
Input/output options
--input [string] Path to comma-separated file containing information about the samples in the experiment.
--outdir [string] The output directory where the results will be saved. You have to use absolute paths to storage on
Cloud infrastructure.
--vector_db [string] Path to directory or tar.gz archive for pre-built PacBio vector database. [default:
${projectDir}/assets/vectorDB.tar.gz]
--email [string] Email address for completion summary.
Reference genome options
--fasta [string] Path to FASTA genome file, either fasta or fasta.gz.
--fai [string] Path to the index file of the FASTA genome file, either fai or gzi.
--align [boolean] Align the input reads to the reference
--interval [string] Interval bed file.
--include_positions [string] Path to a file with a set of sites on the basis of a list of positions to include, Each line of the
input file should contain a (tab-separated) chromosome and position.
--exclude_positions [string] Path to a file with a set of sites on the basis of a list of positions to exclude. Each line of the
input file should contain a (tab-separated) chromosome and position.
Generic options
--help [boolean, string] Display the help message.
--help_full [boolean] Display the full detailed help message.
--show_hidden [boolean] Display hidden parameters in the help message (only works when --help or --help_full are provided).
!! Hiding 16 param(s), use the `--showHidden` parameter to show them !!
------------------------------------------------------
* The pipeline
https://doi.org/10.5281/zenodo.7890527
* The nf-core framework
https://doi.org/10.1038/s41587-020-0439-x
* Software dependencies
https://github.com/sanger-tol/variantcalling/blob/main/CITATIONS.md