Merge pull request #1219 from sanger/pacbio-multiplexing-epic

[EPIC] PacBio multiplexing

app/exchanges/run_csv/deprecated_pacbio_sample_sheet.rb

@@ -42,7 +42,7 @@ def csv_headers
     end
 
     def csv_sample_rows(well)
-      well.libraries.map do |library|
+      well.all_libraries.map do |library|
         # add row under well header for each sample in the well
         csv_data(sample: library, well:, row_type: :sample)
       end

app/models/aliquot.rb

@@ -10,14 +10,15 @@ class Aliquot < ApplicationRecord
 
   belongs_to :tag, optional: true
   belongs_to :source, polymorphic: true
-  belongs_to :well, class_name: 'Pacbio::Well', optional: true, foreign_key: :pacbio_well_id,
-                    inverse_of: :aliquots
+  # Used to identify where a derived aliquot has been used
+  belongs_to :used_by, polymorphic: true, optional: true
 
   # currently I have set these to be validated but not sure
   # as library only validates when a run is created
   # maybe we need to do this when the state is set to used?
   # requests currently dont support these fields so we skip validation on primary aliquots
-  validates :volume, :concentration, :template_prep_kit_box_barcode, :insert_size,
+  # we dont validate insert size as it may not be known at the time of creation
+  validates :volume, :concentration, :template_prep_kit_box_barcode,
             presence: true,
             unless: -> { source.is_a?(Pacbio::Request) && aliquot_type == 'primary' }
   validates :volume, :concentration, :insert_size,

app/models/concerns/aliquotable.rb

@@ -5,15 +5,13 @@ module Aliquotable
   extend ActiveSupport::Concern
 
   included do
-    has_many :aliquots, as: :source, dependent: :nullify
-  end
-
-  def primary_aliquot
-    # Something fundamentally wrong if we have more than one
-    aliquots.find_by(aliquot_type: :primary)
-  end
-
-  def derived_aliquots
-    aliquots.where(aliquot_type: :derived)
+    has_many :aliquots, as: :source, dependent: :destroy
+    has_many :used_aliquots, as: :used_by, dependent: :destroy, class_name: 'Aliquot'
+    has_one :primary_aliquot, -> { where(aliquot_type: :primary) },
+            as: :source, class_name: 'Aliquot',
+            dependent: :destroy, inverse_of: :source
+    has_many :derived_aliquots, -> { where(aliquot_type: :derived) },
+             as: :source, class_name: 'Aliquot',
+             dependent: :nullify, inverse_of: :source
   end
 end

app/models/concerns/sample_sheet.rb

@@ -35,20 +35,20 @@ def barcode_set
 
     # Determines rendering of a row-per sample
     def show_row_per_sample?
-      sample_sheet_behaviour.show_row_per_sample?(libraries)
+      sample_sheet_behaviour.show_row_per_sample?(all_libraries)
     end
 
     # Returns libraries only if they should be shown per row
     def libraries_to_show_per_row
       return unless show_row_per_sample?
 
-      libraries
+      all_libraries
     end
 
     # Sample Name field
-    def pool_barcode
-      # First pool in well's barcode as samples names are already contained in bio sample name
-      pools.first.tube.barcode
+    def tube_barcode
+      # Gets the firsts library barcode which will either be the pool barcode or the library barcode
+      all_libraries.first.tube.barcode
     end
 
     # find the plate given the plate_number

app/models/pacbio/library.rb

@@ -13,17 +13,22 @@ class Library < ApplicationRecord
     include Uuidable
     include Librarian
     include SampleSheet::Library
+    include Aliquotable
 
     validates :volume, :concentration,
               :insert_size, presence: true, on: :run_creation
     validates :volume, :concentration,
               :insert_size, numericality: { greater_than_or_equal_to: 0, allow_nil: true }
 
+    has_many :well_libraries, class_name: 'Pacbio::WellLibrary', foreign_key: :pacbio_library_id,
+                              dependent: :nullify, inverse_of: :library
+    has_many :wells, class_name: 'Pacbio::Well', through: :well_libraries
+
     belongs_to :request, class_name: 'Pacbio::Request', foreign_key: :pacbio_request_id,
                          inverse_of: :libraries
     belongs_to :tag, optional: true
     belongs_to :pool, class_name: 'Pacbio::Pool', foreign_key: :pacbio_pool_id,
-                      inverse_of: :libraries
+                      inverse_of: :libraries, optional: true
     belongs_to :tube, optional: true
 
     has_one :sample, through: :request
@@ -35,6 +40,34 @@ class Library < ApplicationRecord
 
     has_one :source_plate, through: :source_well, source: :plate, class_name: '::Plate'
 
+    # TODO: remove pool constraint this when pools are updated for aliquots
+    validates :primary_aliquot, presence: true, if: -> { pool.blank? }
+    accepts_nested_attributes_for :primary_aliquot, allow_destroy: true
+
+    after_create :create_used_aliquot, :create_tube, if: -> { pool.blank? }
+    before_destroy :check_for_associated_wells, prepend: true
+
+    def create_used_aliquot
+      used_aliquots.create(
+        source: request,
+        aliquot_type: :derived,
+        volume:,
+        concentration:,
+        template_prep_kit_box_barcode:,
+        insert_size:,
+        tag:
+      )
+    end
+
+    def create_tube
+      self.tube = tube || Tube.create!
+      save
+    end
+
+    def tube
+      pool ? pool.tube : super
+    end
+
     def collection?
       false
     end
@@ -43,6 +76,30 @@ def sample_sheet_behaviour
       SampleSheetBehaviour.get(tag_set&.sample_sheet_behaviour || :untagged)
     end
 
-    delegate :sequencing_plates, to: :pool
+    # @return [Array] of Runs that the pool is used in
+    def sequencing_runs
+      wells&.collect(&:run)&.uniq
+    end
+
+    # @return [Array] of Plates attached to a sequencing run
+    def sequencing_plates
+      # TODO: remove this when pools are updated for aliquots
+      if pool
+        pool.sequencing_plates
+      else
+        wells&.collect(&:plate)
+      end
+    end
+
+    private
+
+    def check_for_associated_wells
+      if wells.empty?
+        true
+      else
+        errors.add(:base, 'Cannot delete a library that is associated with wells')
+        throw(:abort)
+      end
+    end
   end
 end

app/models/pacbio/well.rb

@@ -14,11 +14,9 @@ class Well < ApplicationRecord
     has_many :well_pools, class_name: 'Pacbio::WellPool', foreign_key: :pacbio_well_id,
                           dependent: :destroy, inverse_of: :well, autosave: true
     has_many :pools, class_name: 'Pacbio::Pool', through: :well_pools
-
-    has_many :aliquots, foreign_key: :pacbio_well_id, inverse_of: :well, dependent: :destroy,
-                        autosave: true
-    has_many :libraries, through: :pools
-    has_many :tag_sets, through: :libraries
+    has_many :well_libraries, class_name: 'Pacbio::WellLibrary', foreign_key: :pacbio_well_id,
+                              dependent: :destroy, inverse_of: :well, autosave: true
+    has_many :libraries, class_name: 'Pacbio::Library', through: :well_libraries
 
     # pacbio smrt link options for a well are kept in store field of the well
     # which is mapped to smrt_link_options column (JSON) of pacbio_wells table.
@@ -52,15 +50,10 @@ class Well < ApplicationRecord
 
     validates :row, :column, presence: true
 
-    validates :pools, length: {
-      minimum: 1,
-      message: :well_min_pools
-    }
-
     delegate :run, to: :plate, allow_nil: true
 
     def tag_set
-      tag_sets.first
+      all_libraries.collect(&:tag_set).first
     end
 
     def sample_sheet_behaviour
@@ -75,6 +68,11 @@ def summary
       "#{sample_names} #{comment}".strip
     end
 
+    # A collection of all the libraries for a well
+    def all_libraries
+      pools.collect(&:libraries).flatten + libraries
+    end
+
     # collection of all of the requests for a library
     # useful for messaging
     def request_libraries
@@ -84,25 +82,37 @@ def request_libraries
     # a collection of all the sample names for a particular well
     # useful for comments
     def sample_names(separator = ':')
-      libraries.collect(&:request).collect(&:sample_name).join(separator)
+      all_libraries.collect(&:request).collect(&:sample_name).join(separator)
     end
 
     # a collection of all the tags for a well
     # useful to check whether they are unique
     def tags
-      libraries.collect(&:tag_id)
+      all_libraries.collect(&:tag_id)
     end
 
     def pools?
       pools.present?
     end
 
+    def libraries?
+      libraries.present?
+    end
+
     def template_prep_kit_box_barcode
-      pools? ? pools.first.template_prep_kit_box_barcode : ''
+      if pools?
+        pools.first.template_prep_kit_box_barcode
+      elsif libraries?
+        libraries.first.template_prep_kit_box_barcode
+      end
     end
 
     def insert_size
-      pools? ? pools.first.insert_size : ''
+      if pools?
+        pools.first.insert_size
+      elsif libraries?
+        libraries.first.insert_size
+      end
     end
 
     def collection?

app/models/pacbio/well_library.rb

@@ -0,0 +1,13 @@
+# frozen_string_literal: true
+
+module Pacbio
+  # Pacbio::WellLibrary
+  # A well can contain many libraries
+  # A library can belong in many wells
+  class WellLibrary < ApplicationRecord
+    belongs_to :well, class_name: 'Pacbio::Well', foreign_key: :pacbio_well_id,
+                      inverse_of: :well_libraries
+    belongs_to :library, class_name: 'Pacbio::Library', foreign_key: :pacbio_library_id,
+                         inverse_of: :well_libraries
+  end
+end

app/models/tube.rb

@@ -20,9 +20,21 @@ class Tube < ApplicationRecord
   scope :by_barcode, ->(*barcodes) { where(barcode: barcodes) }
   scope :by_pipeline,
         lambda { |pipeline|
-          joins(:container_materials).where(
-            'container_materials.material_type LIKE ?', "#{pipeline.capitalize}::%"
-          )
+          case pipeline
+          when :pacbio
+            left_outer_joins(:pacbio_pools, :pacbio_library, :pacbio_requests).where(
+              'pacbio_pools.id IS NOT NULL OR pacbio_libraries.id IS NOT NULL OR
+               pacbio_requests.id IS NOT NULL'
+            )
+          when :ont
+            left_outer_joins(:ont_pools, :ont_requests).where(
+              'ont_pools.id IS NOT NULL OR ont_requests.id IS NOT NULL'
+            )
+          else
+            joins(:container_materials).where(
+              'container_materials.material_type LIKE ?', "#{pipeline.capitalize}::%"
+            )
+          end
         }
 
   def identifier

app/resources/v1/pacbio/aliquot_resource.rb

@@ -0,0 +1,13 @@
+# frozen_string_literal: true
+
+module V1
+  module Pacbio
+    # AliquotResource
+    class AliquotResource < JSONAPI::Resource
+      model_name '::Aliquot'
+
+      attributes :aliquot_type, :source, :used_by, :state,
+                 :volume, :concentration, :insert_size, :template_prep_kit_box_barcode, :tag_id
+    end
+  end
+end

app/resources/v1/pacbio/library_pool_resource.rb

@@ -0,0 +1,45 @@
+# frozen_string_literal: true
+
+module V1
+  module Pacbio
+    # A temporary PacBio Library resource that returns all libraries
+    # This allows for pool libraries to be retrieved from the pools resource
+    class LibraryPoolResource < JSONAPI::Resource
+      include Shared::RunSuitability
+
+      model_name 'Pacbio::Library'
+
+      attributes :state, :volume, :concentration, :template_prep_kit_box_barcode,
+                 :insert_size, :created_at, :deactivated_at, :source_identifier,
+                 :pacbio_request_id, :tag_id
+
+      has_one :request, always_include_optional_linkage_data: true
+      # If we don't specify the relation_name here, jsonapi-resources
+      # attempts to use_related_resource_records_for_joins
+      # In this case I can see it using container_associations
+      # so seems to be linking the wrong tube relationship.
+      has_one :tag, always_include_optional_linkage_data: true
+      has_one :pool, always_include_optional_linkage_data: true
+      has_one :tube, relation_name: :tube, always_include_optional_linkage_data: true
+      has_one :source_well, relation_name: :source_well, class_name: 'Well'
+      has_one :source_plate, relation_name: :source_plate, class_name: 'Plate'
+
+      has_one :primary_aliquot, always_include_optional_linkage_data: true,
+                                relation_name: :primary_aliquot, class_name: 'Aliquot'
+
+      def self.records_for_populate(*_args)
+        super.preload(source_well: :plate, request: :sample,
+                      tag: :tag_set,
+                      container_material: { container: :barcode })
+      end
+
+      def created_at
+        @model.created_at.to_fs(:us)
+      end
+
+      def deactivated_at
+        @model&.deactivated_at&.to_fs(:us)
+      end
+    end
+  end
+end