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4 | 4 | "attachments": {}, |
5 | 5 | "cell_type": "markdown", |
6 | 6 | "metadata": {}, |
7 | | - "source": [ |
8 | | - "# Analyse Nanostring data in Napari-SpatialData \n" |
9 | | - ] |
| 7 | + "source": "# Analyse Cosmx data in Napari-SpatialData\n" |
10 | 8 | }, |
11 | 9 | { |
12 | 10 | "attachments": {}, |
13 | 11 | "cell_type": "markdown", |
14 | 12 | "metadata": {}, |
15 | | - "source": [ |
16 | | - "This tutorial shows how to load and analyse Nanostring data with the Napari-SpatialData plugin.\n" |
17 | | - ] |
| 13 | + "source": "This tutorial shows how to load and analyze Cosmx data with the Napari-SpatialData plugin.\n" |
18 | 14 | }, |
19 | 15 | { |
20 | 16 | "attachments": {}, |
|
60 | 56 | "attachments": {}, |
61 | 57 | "cell_type": "markdown", |
62 | 58 | "metadata": {}, |
63 | | - "source": [ |
64 | | - "Next, we will download the Nanostring dataset (cosmx_io) for analysis. \n", |
65 | | - "\n", |
66 | | - "If on Windows, download the dataset from https://s3.embl.de/spatialdata/spatialdata-sandbox/cosmx_io.zip.\n", |
67 | | - "\n", |
68 | | - "In on Linux, uncomment the lines below to download the dataset. " |
69 | | - ] |
70 | | - }, |
71 | | - { |
72 | | - "cell_type": "code", |
73 | | - "execution_count": 2, |
74 | | - "metadata": {}, |
75 | | - "outputs": [ |
76 | | - { |
77 | | - "name": "stdout", |
78 | | - "output_type": "stream", |
79 | | - "text": [ |
80 | | - "--2023-05-03 21:11:58-- https://s3.embl.de/spatialdata/spatialdata-sandbox/cosmx_io.zip\n", |
81 | | - "Resolving s3.embl.de (s3.embl.de)... 194.94.45.80\n", |
82 | | - "Connecting to s3.embl.de (s3.embl.de)|194.94.45.80|:443... connected.\n", |
83 | | - "HTTP request sent, awaiting response... 200 OK\n", |
84 | | - "Length: 2210531049 (2,1G) [application/zip]\n", |
85 | | - "Saving to: ‘tutorial_data/nanostring_data/cosmx_io.zip’\n", |
86 | | - "\n", |
87 | | - "cosmx_io.zip 100%[===================>] 2,06G 10,9MB/s in 3m 21s \n", |
88 | | - "\n", |
89 | | - "2023-05-03 21:15:19 (10,5 MB/s) - ‘tutorial_data/nanostring_data/cosmx_io.zip’ saved [2210531049/2210531049]\n", |
90 | | - "\n" |
91 | | - ] |
92 | | - } |
93 | | - ], |
94 | | - "source": [ |
95 | | - "# !mkdir tutorial_data\n", |
96 | | - "# !mkdir tutorial_data/nanostring_data\n", |
97 | | - "# !wget -P tutorial_data/nanostring_data https://s3.embl.de/spatialdata/spatialdata-sandbox/cosmx_io.zip\n", |
98 | | - "# !unzip tutorial_data/nanostring_data/cosmx_io.zip -d tutorial_data/nanostring_data" |
99 | | - ] |
| 59 | + "source": "Please download the CosMx raw data from https://brukerspatialbiology.com/products/cosmx-spatial-molecular-imager/ffpe-dataset/nsclc-ffpe-dataset/, parse it using `spatialdata_io` and save it to the SpatialData Zarr format (you can for instance [use this script](https://github.com/giovp/spatialdata-sandbox/blob/main/cosmx_io/to_zarr.py)).\n" |
100 | 60 | }, |
101 | 61 | { |
102 | 62 | "attachments": {}, |
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