Skip to content

Syntax error loading yaml file #406

@iwanttoeatyo

Description

@iwanttoeatyo

Describe the bug

Hello zUMIs developers, thank you for developing such a great tool, I had a little bug when I was using it, I have smartseq2 data

I was using ’git clone https://github.com/sdparekh/zUMIs.git‘ to download the codebase and then using ’sh zUMIs.sh -c -y smartseq2.yaml‘ to analyze it, and the bug appeared

(zUMIs) cqdgkyb@hpc31:~/software_bioinformatics/zUMIs$ sh zUMIs.sh -c -y smartseq2.yaml
Using miniconda environment for zUMIs!
note: internal executables will be used instead of those specified in the YAML file!
Warning message:
package ‘yaml’ was built under R version 4.4.2
Error: Syntax error loading yaml file
Execution halted
YAML file has an error. Look at the zUMIs_YAMLerror.log or contact developers.

I need your help to fix this bug, thanks!

To Reproduce
YAML file
project: samrt-seq2

sequence_files:
file1:
name: /home/cqdgkyb/daping/fastq/CRR058013_S1_R2_001.fastq.gz
base_definition:
- BC(1-8)
- UMI(9-16)
file2:
name: /home/cqdgkyb/daping/fastq/CRR058013_S1_R1_001.fastq.gz
base_definition:
- cDNA(1-300)

reference:
STAR_index: /home/cqdgkyb/software_bioinformatics/star_index
GTF_file: /home/cqdgkyb/software_bioinformatics/gencode.vM36.annotation.gtf

out_dir: /home/cqdgkyb/daping/output

num_threads: 24
mem_limit: 370

filter_cutoffs:
BC_filter:
num_bases: 1
phred: 20
UMI_filter:
num_bases: 1
phred: 20

barcodes:
barcode_num: ~
barcode_file: ~
automatic: yes
BarcodeBinning: 0
nReadsperCell: 100
demultiplex: yes

counting_opts:
introns: yes
downsampling: '0'
strand: 0
Ham_Dist: 0
velocyto: no
primaryHit: yes
twoPass: no

make_stats: yes
which_Stage: Filtering
samtools_exec: samtools
pigz_exec: pigz
STAR_exec: STAR
Rscript_exec: Rscript
zUMIs_directory: /home/cqdgkyb/software_bioinformatics/zUMIs
read_layout: SE

Screenshots
If applicable, add screenshots to help explain your problem.

Desktop (please complete the following information):

  • OS: Ubuntu
  • Version: 20.04.6 LTS (Focal Fossa)

Additional context
Add any other context about the problem here.

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions