High background mutation of CtoT GtoA mutations

[Error-fre]

I took only read1 of paired-end sequencing for my samples and after running slam-dunk all, I used alleyoop rates to calculate the overall conversion rates. I have around 0.5 conversion rate at AtoG(- strand) and TtoC (+ strand) which is expected with my 1hr labeling. However I got 0.7 conversion rate of CtoT(- strand) and GtoA (+ strand) in all my samples including nolabel control.

Have anyone encountered this before? Is it a mapping problem or sample prep problem?

[t-neumann]

Hm not really so by 0.7 you mean 0.7%? That's quite low to be honest, you can check an example report for reference here