diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1645f7212e9e..37ad1a59cc60 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -18,6 +18,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Write to `stdout` when `--output -` flag is used for `@tailwindcss/cli` ([#15656](https://github.com/tailwindlabs/tailwindcss/pull/15656))
 - _Upgrade (experimental)_: Pretty print `--spacing(…)` to prevent ambiguity ([#15596](https://github.com/tailwindlabs/tailwindcss/pull/15596))
 
+### Changed
+
+- Use more modern `--alpha(color / 50%)` syntax instead of `--alpha(color, 50%)` ([#15665](https://github.com/tailwindlabs/tailwindcss/pull/15665))
+
 ## [4.0.0-beta.9] - 2025-01-09
 
 ### Added
diff --git a/packages/tailwindcss/src/css-functions.test.ts b/packages/tailwindcss/src/css-functions.test.ts
index 128053f46cbf..e3747c7ba601 100644
--- a/packages/tailwindcss/src/css-functions.test.ts
+++ b/packages/tailwindcss/src/css-functions.test.ts
@@ -12,7 +12,7 @@ describe('--alpha(…)', () => {
     expect(
       await compileCss(css`
         .foo {
-          margin: --alpha(red, 50%);
+          margin: --alpha(red / 50%);
         }
       `),
     ).toMatchInlineSnapshot(`
@@ -30,7 +30,7 @@ describe('--alpha(…)', () => {
         }
       `),
     ).rejects.toThrowErrorMatchingInlineSnapshot(
-      `[Error: The --alpha(…) function requires two arguments, e.g.: \`--alpha(var(--my-color), 50%)\`]`,
+      `[Error: The --alpha(…) function requires a color and an alpha value, e.g.: \`--alpha(var(--my-color) / 50%)\`]`,
     )
   })
 
@@ -42,7 +42,7 @@ describe('--alpha(…)', () => {
         }
       `),
     ).rejects.toThrowErrorMatchingInlineSnapshot(
-      `[Error: The --alpha(…) function requires two arguments, e.g.: \`--alpha(red, 50%)\`]`,
+      `[Error: The --alpha(…) function requires a color and an alpha value, e.g.: \`--alpha(red / 50%)\`]`,
     )
   })
 
@@ -50,11 +50,11 @@ describe('--alpha(…)', () => {
     expect(() =>
       compileCss(css`
         .foo {
-          margin: --alpha(red, 50%, blue);
+          margin: --alpha(red / 50%, blue);
         }
       `),
     ).rejects.toThrowErrorMatchingInlineSnapshot(
-      `[Error: The --alpha(…) function only accepts two arguments, e.g.: \`--alpha(red, 50%)\`]`,
+      `[Error: The --alpha(…) function only accepts one argument, e.g.: \`--alpha(red / 50%)\`]`,
     )
   })
 })
diff --git a/packages/tailwindcss/src/css-functions.ts b/packages/tailwindcss/src/css-functions.ts
index bb27658c5929..ff986c6f6590 100644
--- a/packages/tailwindcss/src/css-functions.ts
+++ b/packages/tailwindcss/src/css-functions.ts
@@ -12,20 +12,22 @@ const functions: Record<string, (designSystem: DesignSystem, ...args: string[])
   theme: legacyTheme,
 }
 
-function alpha(_designSystem: DesignSystem, value: string, alpha: string, ...rest: string[]) {
-  if (!value || !alpha) {
+function alpha(_designSystem: DesignSystem, value: string, ...rest: string[]) {
+  let [color, alpha] = segment(value, '/').map((v) => v.trim())
+
+  if (!color || !alpha) {
     throw new Error(
-      `The --alpha(…) function requires two arguments, e.g.: \`--alpha(${value || 'var(--my-color)'}, ${alpha || '50%'})\``,
+      `The --alpha(…) function requires a color and an alpha value, e.g.: \`--alpha(${color || 'var(--my-color)'} / ${alpha || '50%'})\``,
     )
   }
 
   if (rest.length > 0) {
     throw new Error(
-      `The --alpha(…) function only accepts two arguments, e.g.: \`--alpha(${value || 'var(--my-color)'}, ${alpha || '50%'})\``,
+      `The --alpha(…) function only accepts one argument, e.g.: \`--alpha(${color || 'var(--my-color)'} / ${alpha || '50%'})\``,
     )
   }
 
-  return withAlpha(value, alpha)
+  return withAlpha(color, alpha)
 }
 
 function spacing(designSystem: DesignSystem, value: string, ...rest: string[]) {