Hello,
Thank you for creating such an innovative, streamlined package! I am using scvelo in my single cell analyses and had a question regarding the scvelo results I got. When I checked the spliced/unspliced percentages scv.pl.proportions(adata), I got majority unspliced (86%) and minority spliced (14%), which seem way off from the reported 15-25% of unspliced intronic reads in La Manno et al 2018. I was wondering whether these statistics indicate something wrong with my analysis method or I should take this as factual and trust my downstream results. Here's my method: I used velocyto to generate individual loom files for all 9 of my 10x Chromium samples, merged the data, and subset to select 18555 genes x 14813 cells of interest. The downstream analyses presented in scvelo tutorial page all ran successfully.
Thanks so much for the help!
-Angela
Hello,
Thank you for creating such an innovative, streamlined package! I am using
scveloin my single cell analyses and had a question regarding the scvelo results I got. When I checked the spliced/unspliced percentagesscv.pl.proportions(adata), I got majority unspliced (86%) and minority spliced (14%), which seem way off from the reported 15-25% of unspliced intronic reads in La Manno et al 2018. I was wondering whether these statistics indicate something wrong with my analysis method or I should take this as factual and trust my downstream results. Here's my method: I usedvelocytoto generate individual loom files for all 9 of my 10x Chromium samples, merged the data, and subset to select 18555 genes x 14813 cells of interest. The downstream analyses presented inscvelotutorial page all ran successfully.Thanks so much for the help!
-Angela