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| 1 | +# Introduction {.unnumbered} |
| 2 | + |
| 3 | + |
| 4 | +## What is msDiaLogue? |
| 5 | + |
| 6 | + |
| 7 | +**msDiaLogue** is an R package developed through a collaboration between |
| 8 | +the [Proteomics & Metabolomics Facility (PMF)](https://proteomics.uconn.edu/) |
| 9 | +and the [Statistical Consulting Services (SCS)](https://statsconsulting.uconn.edu/) |
| 10 | +at the [University of Connecticut (UConn)](https://uconn.edu/) |
| 11 | +It provides a modular workflow for analyzing untargeted mass spectrometry |
| 12 | +(MS)-based proteomics data, together with a wide range of visualization options |
| 13 | +for both Data-Dependent Acquisition (DDA) and Data-Independent Acquisition (DIA) |
| 14 | +datasets. |
| 15 | + |
| 16 | + |
| 17 | +::: {style="text-align: center;"} |
| 18 | +[{style="width:34%; margin:0 2%"}](https://uconn.edu/) |
| 19 | +[{style="width:30.6%; margin:0 2%"}](https://core.uconn.edu/) |
| 20 | +[{style="width:25.5%; margin:0 0%"}](https://statsconsulting.uconn.edu/) |
| 21 | +::: |
| 22 | + |
| 23 | + |
| 24 | +## Design philosophy |
| 25 | + |
| 26 | + |
| 27 | +Unlike monolithic analysis pipelines, **msDiaLogue** is designed as a modular |
| 28 | +workflow. |
| 29 | +Each analysis step is implemented as an independent module that can be used on |
| 30 | +its own or combined with other modules. |
| 31 | +This design allows users to adapt the workflow to different experimental designs, |
| 32 | +software platforms, and analysis objectives. |
| 33 | + |
| 34 | + |
| 35 | +## Workflow |
| 36 | + |
| 37 | + |
| 38 | +:::: {style="text-align: center;"} |
| 39 | + |
| 40 | +:::: |
| 41 | + |
| 42 | + |
| 43 | +## Organization of this cookbook |
| 44 | + |
| 45 | + |
| 46 | +The cookbook is organized into independent recipes corresponding to the major |
| 47 | +modules of **msDiaLogue**. |
| 48 | +Each chapter introduces one module using practical examples and can be read |
| 49 | +independently. |
| 50 | +Readers may follow the recipes in sequence as a complete workflow or use |
| 51 | +individual chapters as references for specific analysis tasks. |
| 52 | + |
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