How to show all assumption

[zimachen]

Hello, I would like to know how to display all the assumptions. In the figure, the spectrum with scan=239 in adult_cortex_85869.mgf corresponds to two peptides. Only the peptide with rank 1 is shown in the list. Unfortunately, this peptide, after verification by peptide match, can be matched with a classic protein, so it needs to be discarded. The other peptide, after verification by peptide match, cannot be matched with a classic protein, now I want to view its PSM. What should I do?

[KaiLiCn]

Hi,

Based on the file detail information, your result was from MS-GF+. PDV only shows the first hit from MS-GF+ result. If you want to visualize another peptide on the same spectra, there are two possible ways:

1. Click the "Tools" and select "Check peptide" function. Type the new peptide sequence and add modification if you want. Then a mirror spectrum will be shown in the main panel.

2. Export the current spectrum as a mgf file by clicking the "Export" and select "MGF". Then open a new PDV and select the "One PSM" function.

If you also have the result from FragPipe, you could visualize all ranks PSMs for each spectrum.

Best,
Kai

[zimachen]

Thank you very much for your help! The above - mentioned problem has been solved.