diff --git a/.changeset/silent-planes-show.md b/.changeset/silent-planes-show.md
new file mode 100644
index 000000000000..0df1ab5c6e5a
--- /dev/null
+++ b/.changeset/silent-planes-show.md
@@ -0,0 +1,5 @@
+---
+'astro': patch
+---
+
+Fixes a bug where highlights and tooltips render over the audit list window.
diff --git a/packages/astro/e2e/dev-toolbar.test.js b/packages/astro/e2e/dev-toolbar.test.js
index 628b3af3aa80..aa03680942e4 100644
--- a/packages/astro/e2e/dev-toolbar.test.js
+++ b/packages/astro/e2e/dev-toolbar.test.js
@@ -241,7 +241,8 @@ test.describe('Dev Toolbar', () => {
 
 		const auditCanvas = toolbar.locator('astro-dev-toolbar-app-canvas[data-app-id="astro:audit"]');
 		const auditHighlights = auditCanvas.locator('astro-dev-toolbar-highlight');
-		for (const highlight of await auditHighlights.all()) {
+		const highlights = (await auditHighlights.all()).filter((_, index) => index !== 1);
+		for (const highlight of highlights) {
 			await expect(highlight).toBeVisible();
 			await highlight.hover();
 			const tooltip = highlight.locator('astro-dev-toolbar-tooltip');
@@ -256,6 +257,22 @@ test.describe('Dev Toolbar', () => {
 		}
 	});
 
+	test('tooltip is rendered behind audit list window', async ({ page, astro }) => {
+		await page.goto(astro.resolveUrl('/tooltip-position'));
+
+		const toolbar = page.locator('astro-dev-toolbar');
+		const appButton = toolbar.locator('button[data-app-id="astro:audit"]');
+		await appButton.click();
+
+		const auditCanvas = toolbar.locator('astro-dev-toolbar-app-canvas[data-app-id="astro:audit"]');
+		const auditHighlights = auditCanvas.locator('astro-dev-toolbar-highlight');
+		const highlight = auditHighlights.nth(1)
+
+		await expect(async() => {
+			await highlight.hover({timeout: 100});
+		}).rejects.toThrowError()
+	});
+
 	test('can open Settings app', async ({ page, astro }) => {
 		await page.goto(astro.resolveUrl('/'));
 
diff --git a/packages/astro/src/runtime/client/dev-toolbar/apps/audit/ui/audit-list-window.ts b/packages/astro/src/runtime/client/dev-toolbar/apps/audit/ui/audit-list-window.ts
index 472e8d7c6409..a87cad395cd3 100644
--- a/packages/astro/src/runtime/client/dev-toolbar/apps/audit/ui/audit-list-window.ts
+++ b/packages/astro/src/runtime/client/dev-toolbar/apps/audit/ui/audit-list-window.ts
@@ -83,7 +83,7 @@ export class DevToolbarAuditListWindow extends HTMLElement {
 					"Noto Color Emoji";
 				color: rgba(191, 193, 201, 1);
 				position: fixed;
-				z-index: 999999999;
+				z-index: 2000000009;
 				bottom: 72px;
 				left: 50%;
 				transform: translateX(-50%);