@@ -36,10 +36,10 @@ times aligned records intersect known gene annotations.
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```
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Gene expression quantification
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- Usage: squab quantify [OPTIONS] --output <OUTPUT> -- annotations <ANNOTATIONS> <BAM >
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+ Usage: squab quantify [OPTIONS] --annotations <ANNOTATIONS> <SRC >
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Arguments:
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- <BAM > Input alignment file
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+ <SRC > Input alignment file
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Options:
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--with-secondary-records
@@ -57,13 +57,13 @@ Options:
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--min-mapping-quality <MIN_MAPPING_QUALITY>
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[default: 10]
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-o, --output <OUTPUT>
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- Output destination for feature counts
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+ Output destination
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-a, --annotations <ANNOTATIONS>
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Input annotations file (GFF3)
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--threads <THREADS>
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Force a specific number of threads
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-h, --help
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- Print help information
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+ Print help (see more with '--help')
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```
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The default output is a tab-delimited text file with two columns: the feature
@@ -84,37 +84,18 @@ using `squab quantify` or `htseq-count`.
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```
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Normalize features counts
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- Usage: squab normalize [OPTIONS] --annotations <ANNOTATIONS> <COUNTS >
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+ Usage: squab normalize [OPTIONS] --annotations <ANNOTATIONS> <SRC >
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Arguments:
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- <COUNTS>
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- Input counts file
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+ <SRC> Input counts file
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Options:
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- -t, --feature-type <FEATURE_TYPE>
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- Feature type to count
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-
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- [default: exon]
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-
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- -i, --id <ID>
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- Feature attribute to use as the feature identity
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-
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- [default: gene_id]
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-
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- -a, --annotations <ANNOTATIONS>
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- Input annotations file (GFF3)
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-
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- --method <METHOD>
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- Quantification normalization method
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-
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- [default: tpm]
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-
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- Possible values:
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- - fpkm: fragments per kilobase per million mapped reads
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- - tpm: transcripts per million
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-
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- -h, --help
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- Print help information (use `-h` for a summary)
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+ -t, --feature-type <FEATURE_TYPE> Feature type to count [default: exon]
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+ -i, --id <ID> Feature attribute to use as the feature identity [default: gene_id]
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+ -a, --annotations <ANNOTATIONS> Input annotations file (GFF3)
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+ --method <METHOD> Quantification normalization method [default: tpm] [possible values: fpkm, tpm]
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+ -o, --output <OUTPUT> Output destination
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+ -h, --help Print help (see more with '--help')
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```
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The output is a tab-delimited text file with two columns: the feature
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