High-throughput screening of In silico PCR
This bash script aims to use Egon's perl script with hunderds or thousands of genomes. Absolutely, you should have good computer (speaking of RAMs) to do it!!
Make sure that you have mafft (install it via conda or apt) !
Also, you should have the (*.fna) genomes files in the same directory as the perl and the bash script !
Type in your command line, then just answer questions !
bash HTS_PCR.shYou will get unaligned amplicons and aligned amplicons with a tree
Everything is clear right? But anyhow, contact here or directly via email : drahmedsherbini@yahoo.com
This tool aims to help others. Kindly, cite Egon and my github page !