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Fix section header to BICCN, rename Corces2018→Granja2021, verify all refs
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paper/PyPR.png

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paper/paper.bib

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@@ -20,7 +20,7 @@ @article{Zhang2008
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doi = {10.1186/gb-2008-9-9-r137}
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}
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@article{Corces2018,
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@article{Granja2021,
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author = {Granja, Jeffrey M and Corces, M Ryan and Pierce, Sarah E and Bagdatli, S Tansu and Choudhry, Hani and Chang, Howard Y and Greenleaf, William J},
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title = {{ArchR} is a scalable software package for integrative single-cell chromatin accessibility analysis},
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journal = {Nature Genetics},

paper/paper.md

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@@ -70,7 +70,7 @@ identify open chromatin regions but rank peaks only by fold change or p-value,
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reflecting signal strength rather than cell-type specificity — a peak with
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high fold change may be active across many cell types and therefore a poor
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candidate for cell-type targeted AAV tools. Differential accessibility tools
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such as ArchR [@Corces2018] test for cell-type enrichment but operate within
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such as ArchR [@Granja2021] test for cell-type enrichment but operate within
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their own data model and do not produce portable, tool-agnostic feature tables.
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Annotation tools such as GREAT [@McLean2010] link peaks to genes but do not
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score chromatin features. At the library level, `pyBigWig` [@Ramirez2020pyBigWig]
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(kurtosis, skewness, bimodality). Figure adapted from Wirthlin et al.
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(2026) [@Wirthlin2026].](figure6_panelA.png){ width=100% }
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# Ranking vs. MACS2 fold change
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# Benchmarking against the BICCN challenge
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Sorting peaks by MACS2 [@Zhang2008] fold change ranks by signal strength
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but not specificity. ArchR [@Corces2018] ranks by differential accessibility
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but not specificity. ArchR [@Granja2021] ranks by differential accessibility
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(log2FC, FDR), capturing enrichment but without normalising signal across
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all background groups.
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Figure 2 illustrates these differences using three validated cortical
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Figure 2 illustrates how PyPeakRankR's specificity metric differs from
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signal-magnitude approaches using three validated cortical
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enhancers from the Brain Initiative Cell Census Network (BICCN) Community
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Challenge [@Johansen2025], where PyPeakRankR ranked third among 16 methods.
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All three peaks target L5 extratelencephalic (ET) neurons in mouse motor

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