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v3.2.0#278

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williamrowell merged 4 commits intomainfrom
develop-v3
Jan 15, 2026
Merged

v3.2.0#278
williamrowell merged 4 commits intomainfrom
develop-v3

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  • Upgrade to TRGT 5.0.0
  • Move TRGT task downstream of phasing, resulting in slightly shorter but much more accurate phase blocks. TRGT genotypes are still in phase with small variants, structural variants, and BAM haplotags.
  • Replaced the script used to find TRGT locus dropouts. find_trgt_dropouts.py more accurately detects coverage dropouts at TRGT repeat catalog loci in a small fraction of the time. The output columns for *.trgt.dropouts.txt are now:
    • chrom
    • start
    • end
    • trid
    • expected_ploidy
    • hap1_count
    • hap2_count
    • unphased_count
    • fail_read_count
    • dropout (FullDropout, HaplotypeDropout, PhasingDropout)

williamrowell and others added 3 commits December 19, 2025 09:46
* bump: Update TRGT to v5.0.0

- move trgt from upstream of phasing to downstream
- updated docs and DAGs
- updated image manifest

* feat: Replace check_trgt_coverage.py with find_trgt_dropouts.py

- new script runs in ~2m for 1M sites, and uses trgt.spanning.sorted.bam, expected ploidy bed, and trgt_catalog as input
- removed coverage_dropouts task and run within trgt task
- DeepVaraint is the subworkflow in upstream.wdl with the longest walltime.
- DeepVariant subworkflow can accept an array of aBAMs.
- Merging aBAMs typically takes ~30 minutes, so passing the array to DeepVariant  shaves ~30 minutes off of upstream.wdl walltime.
@williamrowell williamrowell self-assigned this Jan 15, 2026
@williamrowell williamrowell merged commit 3469fa0 into main Jan 15, 2026
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2 participants