Opentron Setup and Protocol Developments
- Task: Write protocols for the OpenTrons OT2 liquid handling robot for RNA extraction using various commercial and open-source kits
- Location: Open Cell; Contact: Helene at +447400277072
Station A: Sample Plating (needs to be in a separate room)
- p20 + p300 single-channel pipettes
- Temperature Module (actively cool control RNA)
- 4x tube racks
- 96 Deepwell Extraction Plate
- 1x rack 20ul + 1x rack 200ul filter tips
Station B: RNA Extraction (potentially needs to be placed in a BSL cabinet)
- p300 8-channel pipette
- Magnetic Module
- 3x 96 Deepwell Plate
- 96 Well Elution Plate
- 5x racks of 200ul filter tips
- 1x liquid trash reservoirs
Station C - Assay Setup (next to qPCR machine) - extra clean environment!
- p20 single-channel
- Opentrons Temperature Module
- 96 Well Elution Plate
- 96 Well PCR Plate
- 3x racks 20ul filter tips
Link to set-up
OpenTrons Blog: testing-for-covid-19-with-opentrons
- Inventory list for the set-up Google sheet
- Action Sequence Google doc