optgene.run() not working with growth_coupled=True

[jafemm]

Hi!

I'm trying to run the following code:

from cameo.strain_design.heuristic.evolutionary_based import OptGene

result4 = optgene.run(target="EX_btd_RR_e", 
                     biomass="BIOMASS_Ecoli_core_w_GAM",
                     substrate="glc__D_e",
                     max_evaluations=20000, population_size=200, max_knockouts=10,
                     plot=False, growth_coupled = True)

But when I run this, it starts loading some points on the graph but then I receive the following error:

When I do it without the growth_coupled option it works fine. I'm not sure what the problem could be.

Im working with the E. coli core model and I added the reacctions and metabolites that let the model produce EX_btd_RR_e.
The lower bound for the E. coli core biomass is set to 0 as well as the lower boud for EX_btd_RR_e.

Thanks in advanced,

Javiera