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README.md

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_A lightweight, human-in-the-loop microscopy image analysis app._
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**[Homepage](https://biosustain.github.io/mycol/)**  ·  [FAQ](https://biosustain.github.io/mycol/faq.html)  ·  [Functionality Guide](https://biosustain.github.io/mycol/functionality.html)
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**[Homepage](https://biosustain.github.io/mycol/)**  ·  [FAQ](https://biosustain.github.io/mycol/faq.html)  ·  [Functionality Guide](https://biosustain.github.io/mycol/functionality.html)  ·  [Preprint](https://www.biorxiv.org/content/10.64898/2026.06.02.729113v1)
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</div>
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- Automating image annotation (with human QC)
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- Morphology-based phenotypic comparison
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Each example below was segmented in Mycol and exported with its mask overlay. Where the study defines classes, cells are coloured by class; where it does not, masks take the unlabelled colour.
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<table>
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<tr>
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<td width="38%" valign="middle">
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**Spore germination**
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Every cell segmented, then classified as **Germinated** (red) or **Ungerminated** (blue) - 27 and 35 respectively in this frame.
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</td>
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<td width="62%">
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<img src="docs/case_study_examples/cs1_spore_germination.png" alt="Spore germination example, cells coloured by germination class" width="100%" />
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</td>
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</tr>
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<tr>
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<td width="38%" valign="middle">
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**Larval morphology**
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Larvae segmented and classified as **normal** (blue) or **abnormal** (red), so shape descriptors can be compared between the two groups.
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</td>
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<td width="62%">
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<img src="docs/case_study_examples/cs2_larvae_morphology.png" alt="Larval morphology example, larvae coloured by normal or abnormal class" width="100%" />
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</td>
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</tr>
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<tr>
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<td width="38%" valign="middle">
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**Colony (CFU) counting**
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All 734 colonies on this plate segmented in one pass. No classes are defined here, so every mask is drawn in the unlabelled colour.
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</td>
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<td width="62%">
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<img src="docs/case_study_examples/cs3_cfu_counting.png" alt="Agar plate with every colony segmented" width="100%" />
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</td>
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</tr>
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</table>
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## License
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docs/functionality.html

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<a href="index.html" class="badge">Workflow</a>
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<a href="faq.html" class="badge">FAQ</a>
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<a href="functionality.html" class="badge current">Functionality Guide</a>
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<a href="https://www.biorxiv.org/content/10.64898/2026.06.02.729113v1" target="_blank" rel="noopener" class="badge">Preprint</a>
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<a href="https://github.com/biosustain/mycol" target="_blank" rel="noopener" class="badge">GitHub</a>
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</div>
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</section>

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