Phylogenetic community structure and stable isotope analysis of the parasitoid community associated with Eastern spruce budworm (Lepidoptera: Tortricidae)

Authors

Christopher J. Greyson-Gaito^*1, Sarah J. Dolson², Glen Forbes³, Rosanna Lamb ³, Wayne E. MacKinnon³, Kevin S. McCann¹, M. Alex Smith¹, Eldon S. Eveleigh^3,4

Affiliations

*Corresponding author - christopher@greyson-gaito.com

1. Department of Integrative Biology, University of Guelph, Guelph, ON, Canada
2. Department of Biometry and Enviornmental System Analysis, Albert-Ludwigs-Universität Freiburg, Freiburg, Germany
3. Natural Resources Canada, Canadian Forest Service, Atlantic Foresty Centre, Fredericton, New Brunswick, Canada
4. Population Ecology Group, Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, New Brunswick, Canada

ORCID

• CJGG – 0000-0001-8716-0290
• SJD – 0000-0001-9312-2282
• KSM – 0000-0001-6031-7913
• MAS – 0000-0002-8650-2575
• ESE – 0000-0001-5060-8565

Dataset and R script containing statistical analysis/figure creation.

Instructions

1. Download the GitHub/Zenodo repo
2. Open RStudio, and open the project called SpruceBudworm_Parasitoids_Hardwood.Rproj
3. Then open the R file called Hardwood_SBW_GreysonGaitoetal2021_RScript.R.

Because you have opened a R project, your working directory has been set to this folder and you can immediately start running the code.

If you do not use RStudio, open the R file Hardwood_SBW_GreysonGaitoetal2021_RScript.R, the normal way you would. Then change your working directory to this folder. You will need to change the paths to the dataset and the figures in the R script.

Folder and file structure

• data
  • SI_data_GreysonGaitoetal2021.csv
  • malaise2016_reared2015_barcoded_metadata_GreysonGaitoetal2021.csv
  • malaise2016_reared2015_barcoded_tree_GreysonGaitoetal2021.nwk
  • reared1980s_barcoded_metadata_GreysonGaitoetal2021.csv
  • reared1980s_barcoded_tree_GreysonGaitoetal2021.nwk
• figs – folder to place figures when created
• .gitignore – file containing files and folders that git should ignore
• Hardwood_SBW_GreysonGaitoetal2021_RScript.R – R script with statistical analyses and figure creation
• LICENSE.txt – CC by 4.0 License for this repository
• README.md – Readme file
• SpruceBudworm_Parasitoid_Hardwood.Rproj – Opens R project
• metadata_SpruceBudworm_Parasitoids_Hardwood_GreysonGaitoetal2021.docx – contains information on each datafile