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GTEx Pro Pipeline — Web Application Version

Note

For full functionality and robust preprocessing, please use the GTEx Pro Web Application:
👉 https://multi-tissue-rnaseq.lovable.app/


Overview

GTEx Pro provides a powerful, user-friendly interface for high-quality GTEx multi-tissue gene expression analysis.
This web platform replaces the need to run pipeline code locally, offering automated preprocessing, normalization, and batch correction directly in the browser.

The academic web version is provided free of charge for limited analysis. All backend processing uses the same validated methods from the original pipeline, ensuring precision and reproducibility.


Features

  • Upload raw read counts and select gene IDs and tissues directly in the interface
  • Automated robust normalization using TMM + CPM
  • Batch correction using SVA
  • Clean visualizations and downloadable expression matrices
  • No installation required — everything runs in the cloud
  • Built-in feedback portal for user suggestions

How to Use

  1. Visit https://multi-tissue-rnaseq.lovable.app/
  2. Upload your raw expression data and metadata files
  3. Choose tissues and provide your genes of interest
  4. Run preprocessing and download your outputs
  5. Provide feedback inside the app to help improve the platform

Demo Video

Check out the demo of the Multi-tissue RNA-seq Analyser:

Watch the demo video


Citation

If you use GTEx Pro for academic work, please cite:

Jothi, D. GTEx Pro enables accurate multi-tissue gene expression analysis using robust normalization and batch correction. Sci Rep 15, 32684 (2025). https://doi.org/10.1038/s41598-025-20697-0.


License / Usage Restrictions

  • Free for academic research use for limited set of analysis
  • Redistribution or modification for commercial purposes is strictly prohibited

Important Note

All pipeline code has been removed from this repository.
The webapp is now the only supported interface for running GTEx Pro analysis.

To request new features or report bugs, please use the feedback option inside the webapp.


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A pre-processing pipeline (normalization+batch correction) for GTEx data

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