OstEoCAR: A Single-Cell Atlas for Osteosarcoma

Preprint doi: https://doi.org/10.64898/2026.01.13.698472

OstEoCAR is a comprehensive single-cell RNA sequencing (scRNA-seq) atlas designed to facilitate cell type annotation and transcriptomic exploration of osteosarcoma across multiple models, including human, xenograft mice, syngeneic mice, and canine samples.

🔬 About OsteoCAR

• Purpose: Provides a harmonized reference for annotating cell types in osteosarcoma datasets.
• Scope: Integrates data from NIH, St. Jude, Nationwide Children's Hospital, Tufts, Colorado State University, University of Minnesota, and public datasets.
• Applications: Supports researchers in identifying tumor subpopulations, stromal interactions, and therapeutic targets.

🚀 Key Features

• Curated Cell Type Annotations: Enables precise classification of osteosarcoma and microenvironmental cells.
• Cross-Species Comparisons: Facilitates comparative transcriptomics across human and model systems.
• Interactive Exploration: Designed for seamless integration with computational tools for visualization and analysis.

📊 How to Use

• Load and analyze single-cell RNA-seq datasets using Seurat, Scanpy, or other bioinformatics pipelines.
• Use the atlas for benchmarking, differential expression analysis, and pathway enrichment studies.
• Explore osteosarcoma heterogeneity, immune interactions, and therapeutic vulnerabilities.

🤝 Contributing

We welcome collaborations to expand, refine, and apply OsteoCAR to new datasets and cancer models.

📩 Contact

For inquiries, collaborations, or access to the dataset, please reach out.
email: budhathoki.3@osu.edu