Serum and CSF metabolomics analysis shows Mediterranean Ketogenic Diet mitigates risk factors of Alzheimer’s disease
This repository contains scripts for analysis and figure generation of the paper
Schweickart*, Batra* et. al. Serum and CSF metabolomics analysis shows Mediterranean Ketogenic Diet mitigates risk factors of Alzheimer’s disease (2024), npj Metabolic Health and Disease. link
| Script name | Description |
|---|---|
| custom_functions.R | Internal customized functions. Will be sourced by other scripts |
| 1_serum_dataprocessing.R | BEAM serum metabolomics preprocessing |
| 2_serum_association_analysis.R | BEAM serum association analysis |
| 3_csf_dataprocessing.R | BEAM CSF metabolomics preprocessing |
| 4_csf_association_analysis.R | BEAM CSF association analysis |
| 5_metabolome_figures.R | Figures 2a, 2b, 2d, 3a, 4 (Figures 1, 3b, and 4 (partially) were manually generated) |
| 6a_joint-tensor-nightingale-microbiome.ipynb | Microbiome analysis |
| 6b_joint_tensor_factorization_microbiome_nightingale.R | Microbiome-metabolme joint analysis |
| 6c_microbiome_figures.R | Figure 2c |
| Folder | Description |
| input | Contains the input data to be used by the scripts |
| results | Will contain output files |
| Output files in results/ | Description |
| tmp* | Intermediate files used in follow-up scripts |
| supplementary* | Supplementary files from the paper |
| Figure* | Figure panels from the paper |
Input files for scripts: tmp_annotations_sig_serum_diet.csv is provided with content in the input folder.
Run scripts in sequence 1 to 5 for metabolomics analysis and figures. Run scripts 6a, 6b, 6c for microbiome analysis and figures.