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stPipe

The stPipe package provides a comprehensive pipeline for preprocessing sequencing-based spatial transcriptomics data, including 10X Visium, BGI Stereo-seq, Slide-seq, and Curio-seeker.

The latest development version can be installed with:

if (!require("devtools", quietly = TRUE))
    install.packages("devtools")
devtools::install_github("mritchielab/stPipe")

or

if (!require("remotes", quietly = TRUE))
    install.packages("remotes")
remotes::install_github("mritchielab/stPipe")

or

if (!requireNamespace("BiocManager", quietly=TRUE))
    install.packages("BiocManager")
BiocManager::install("stPipe")

The sample data is downsampled from a 10X Visium mouse spleen sample (Sample 709). It is a probe-based, FFPE tissue dataset generated from paired-end FASTQ files. The data can be downloaded from Zenodo, and a detailed sample description is available in the SpatialBench paper.

Sample HTML report for demo data can be found here.

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