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Release 4.0.0#207

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Aratz merged 106 commits into
masterfrom
dev
May 25, 2026
Merged

Release 4.0.0#207
Aratz merged 106 commits into
masterfrom
dev

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@Aratz Aratz commented May 19, 2026

[4.0.0] - 2026-05-19

Added

  • Add Proxiome V2 workflow support, enabling sample pooling and processing of up to 8,000 cells per run by @Aratz #204, #205

Enhancements & fixes

  • Use nextflow strict syntax by @Aratz #194
  • Use TMPDIR when defined to store temporary files by @Aratz #195
  • Retry experiment summary up to two times after failure by @Aratz #196
  • Updated pixelatorES to 0.10.1 in PNA experiment summary step by @Aratz #197, #204
  • Add cells_1k and cells_8k Nextflow profiles with process-specific resource overrides for different input scales by @johandahlberg #203
  • Fix singularity CI tests by @Aratz #208
  • Update nf-core template to 4.0.2 by @Aratz #202

Software dependencies

Dependency Old version New version
pixelatorES 0.6.0 0.10.1
pixelator 0.23.0 0.26.0

Warning

Panel and design names have been completely renamed in pixelator 0.26 (nf-core/pixelator 4.0 and above). Refer to the pixelator changelog for more details.

Parameters

Old parameter New parameter
--leiden-iterations
--min-component-size-in-refinement
--min-component-size-to-prune
--edge_cycle-verification

Note

Parameter has been updated if both old and new parameter information is present.
Parameter has been added if just the new parameter information is present.
Parameter has been removed if new parameter information isn't present.

Aratz and others added 30 commits March 4, 2026 10:21
Merge changes from patch 3.0.1 back into dev
As far as I understand, this was set so that Nextflow would re-run in
case the samplesheet changes. Nextflow does this natively though
Make nf-core/pixelator compliant with strict syntax
Co-authored-by: Johan Dahlberg <johan@uppsala-bioinformatics.se>
Use $TMPDIR to set temporaryr directories
Enable retry for experiment summary
@Aratz Aratz requested a review from vagkaratzas May 22, 2026 13:39
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Thanks for the updates! Whatever was left, note down for the next release ;)

@Aratz Aratz merged commit 2f6ec18 into master May 25, 2026
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4 participants