- "help_text": "Use `unmapped` (default) to screen reads rejected by STAR/HISAT2 — requires alignment. Use `trimmed` to screen reads that passed BBSplit and rRNA filtering before alignment. Use `trim_only` to screen reads after adapter trimming but before BBSplit/rRNA removal — recommended when BBSplit removes expected contaminants (e.g. EBV with a mouse-only BBSplit index). Use `raw` to screen pre-trimming reads (adapter sequences still present). `unmapped` requires `--aligner star_salmon`, `--aligner star_rsem`, or `--aligner hisat2`.",
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