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{% if is_nfcore -%}

{{ name }}

{% else -%}

{{ name }}

{% endif -%} {% if github_badges -%} {% if codespaces %}[Open in GitHub Codespaces](https://github.com/codespaces/new/{{ name }}){% endif %} [![GitHub Actions CI Status](https://github.com/{{ name }}/actions/workflows/nf-test.yml/badge.svg)](https://github.com/{{ name }}/actions/workflows/nf-test.yml) [![GitHub Actions Linting Status](https://github.com/{{ name }}/actions/workflows/linting.yml/badge.svg)](https://github.com/{{ name }}/actions/workflows/linting.yml){% endif -%} {% if is_nfcore -%}[AWS CI](https://nf-co.re/{{ short_name }}/results){% endif -%} {%- if github_badges -%} Cite with Zenodo nf-test

Nextflow [![nf-core template version](https://img.shields.io/badge/nf--core_template-{{ nf_core_version }}-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/{{ nf_core_version }}) run with conda run with docker run with singularity [Launch on Seqera Platform](https://cloud.seqera.io/launch?pipeline=https://github.com/{{ name }})

{% endif -%} {%- if is_nfcore -%}[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23{{ short_name }}-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/{{ short_name }}){% endif -%} {%- if is_nfcore -%}Follow on Bluesky{% endif -%} {%- if is_nfcore -%}Follow on Mastodon{% endif -%} {%- if is_nfcore -%}Watch on YouTube

{% endif -%}

Introduction

{{ name }} is a bioinformatics pipeline that ...

{%- if fastqc %}1. Read QC (FastQC){% endif %} {%- if multiqc %}2. Present QC for raw reads (MultiQC){% endif %}

Usage

Note

If you are new to Nextflow and nf-core, please refer to this page on how to set-up Nextflow. {% if test_config %}Make sure to test your setup with -profile test before running the workflow on actual data.{% endif %}

Now, you can run the pipeline using:

nextflow run {{ name }} \
   -profile <docker/singularity/.../institute> \
   --input samplesheet.csv \
   --outdir <OUTDIR>

Warning

Please provide pipeline parameters via the CLI or Nextflow -params-file option. Custom config files including those provided by the -c Nextflow option can be used to provide any configuration except for parameters; see docs.

{% if is_nfcore -%}

For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/{{ short_name }}/usage) and the [parameter documentation](https://nf-co.re/{{ short_name }}/parameters).

Pipeline output

To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/{{ short_name }}/results) tab on the nf-core website pipeline page. For more details about the output files and reports, please refer to the [output documentation](https://nf-co.re/{{ short_name }}/output).

{% endif -%}

Credits

{{ name }} was originally written by {{ author }}.

We thank the following people for their extensive assistance in the development of this pipeline:

Contributions and Support

If you would like to contribute to this pipeline, please see the contributing guidelines.

{% if is_nfcore -%} For further information or help, don't hesitate to get in touch on the [Slack #{{ short_name }} channel](https://nfcore.slack.com/channels/{{ short_name }}) (you can join with this invite).

{% endif -%}

Citations

{% if citations %}

An extensive list of references for the tools used by the pipeline can be found in the CITATIONS.md file. {%- endif %}

{% if is_nfcore -%} You can cite the nf-core publication as follows:

{% else -%} This pipeline uses code and infrastructure developed and maintained by the nf-core community, reused here under the MIT license.

{% endif -%}

The nf-core framework for community-curated bioinformatics pipelines.

Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.

Nat Biotechnol. 2020 Feb 13. doi: 10.1038/s41587-020-0439-x.