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Update neoantigen.md
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learning-pathways/neoantigen.md

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- subinamehta
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pathway:
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- section: "Module 1: Fusion-Database Generation"
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- section: "Module 1a: Fusion-Database Generation"
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description: |
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Learn how to generate a personalized fusion peptide database using RNA-seq data. This step sets the foundation for identifying tumor-specific fusion peptides in downstream analyses.
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tutorials:
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- name: neoantigen-1-fusion-database-generation
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topic: proteomics
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- section: "Module 2: Non-Reference Database Generation"
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- section: "Module 1b: Non-Reference Database Generation"
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description: |
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Construct a non-reference proteogenomic database incorporating somatic mutations, indels, and other genomic alterations from VCF data.
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tutorials:
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- name: neoantigen-2-non-reference-database-generation
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topic: proteomics
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- section: "Module 3: Database Merge and FragPipe Discovery"
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- section: "Module 2: Database Merge and FragPipe Discovery"
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description: |
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Merge the fusion and non-reference databases, then use FragPipe for mass spectrometry-based discovery of putative neopeptides.
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tutorials:
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- name: neoantigen-3-fragpipe-discovery
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topic: proteomics
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- section: "Module 4: PepQuery2 Verification"
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- section: "Module 3: PepQuery2 Verification"
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description: |
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Perform targeted verification of neoantigen candidates using PepQuery2 for peptide-spectrum match validation.
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tutorials:
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- name: neoantigen-4-peptide-verification
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topic: proteomics
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- section: "Module 5: Variant Annotation"
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- section: "Module 4: Variant Annotation"
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description: |
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Annotate validated neopeptides with their corresponding genomic variants and protein context.
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tutorials:
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- name: neoantigen-5-variant-annotation
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topic: proteomics
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- section: "Module 6: Predicting HLA Binding"
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- section: "Module 5a: Predicting HLA Binding"
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description: |
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Predict MHC binding affinity of validated neopeptides using tools such as NetMHCpan or similar.
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tutorials:
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- name: neoantigen-6-predicting-hla-binding
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topic: proteomics
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- section: "Module 7: IEDB Binding of PepQuery Validated Neopeptides"
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- section: "Module 5b: IEDB Binding of PepQuery Validated Neopeptides"
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description: |
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Assess the immunogenic potential of neopeptides by checking their binding predictions against immune epitope databases such as IEDB.
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tutorials:

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