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name: cellranger_count_split
namespace: mapping
description: Split 10x Cell Ranger output directory into separate output fields.
authors:
- __merge__: /src/authors/angela_pisco.yaml
roles: [ author ]
- __merge__: /src/authors/samuel_d_souza.yaml
roles: [ author ]
- __merge__: /src/authors/robrecht_cannoodt.yaml
roles: [ author, maintainer ]
arguments:
- name: --input
type: file
required: true
example: input_dir
description: Output directory from a Cell Ranger count run.
- name: --filtered_h5
description: |
Output path for the h5 file storing the filtered counts.
type: file
direction: output
required: false
example: filtered_feature_bc_matrix.h5
- name: --metrics_summary
description: |
Where to store the 'metrics_summary' CSV file.
type: file
direction: output
required: false
example: metrics_summary.csv
- name: --molecule_info
description: |
Where to store Cell Ranger's 'molecule_info.h5' file.
type: file
direction: output
required: false
example: molecule_info.h5
- name: --bam
description: |
Location of output BAM files.
type: file
direction: output
required: false
example: possorted_genome_bam.bam
- name: --bai
description: |
Where to store the BAM index files.
type: file
direction: output
required: false
example: possorted_genome_bam.bam.bai
- name: --raw_h5
description: |
Output path for the h5 file storing the raw counts.
type: file
direction: output
required: false
# description: Counts in AnnData format.
example: raw_feature_bc_matrix.h5
resources:
- type: bash_script
path: script.sh
engines:
- type: docker
image: "ubuntu:jammy"
setup:
- type: docker
run: apt update && apt upgrade -y
runners:
- type: executable
- type: nextflow