DE with unintegrated data

[planti-body]

I am performing differential gene expression analysis on a (treatment/control) dataset that has not been integrated, only SCTransformed.
In this case, do I still need to revert to the "RNA" assay and run "NormalizeData" before performing FindAllMarkers, FindMarkers, and FindConservedMarkers? Or can I remain on the "SCT" assay?

I've only been encountering this question in terms of integrated data, so I'm confused about my scenario