Integration multiple samples based on three variables (time, treatment, biological replicate) and how to use split_by

[TiphaineCMartin]

Hi,

I would like to integrate several samples and then use time to time the function split to make difference visualization
I have 3 variables: Time treatment, biological replicate
How can I put this information association with each sample and I can use one or two or three as way to split visualization or analysis?

I did like that
ifnb.list <- list("day0_none_rep1"=seurat_object_day0_rep1, "day0_none_rep2"=seurat_object_day0_rep2, "day1_ctrl_rep1"=seurat_object_day1_ctrl_rep1, "day1_ctrl_rep2"=seurat_object_day1_ctrl_rep2, "day1_vpa_rep1"=seurat_object_day1_vpa_rep1, "day1_vpa_rep2"=seurat_object_day1_vpa_rep2, "day2_vpa_rep1"=seurat_object_day2_vpa_rep1, "day2_vpa_rep2"=seurat_object_day2_vpa_rep2 )

but I don't think that it is good

thanks,
Tiphaine