identifying DEGs with integrated data

[GreyRockIQ]

Hello
I have errors with identifying DEGs with integrated data as below with code

t.cells <- subset(immune.combined, idents = "CD4 Naive T")
Idents(t.cells) <- "stim"
avg.t.cells <- as.data.frame(log1p(AverageExpression(t.cells, verbose = FALSE)$RNA))
avg.t.cells$gene <- rownames(avg.t.cells)

Errors as Couldn't find functionAverageExpression

Any suggestions?
Thanks
GreyRock

[samuel-marsh]

Hi @GreyRockIQ

Have you run library(Seurat) at the start of your R session?

Best,
Sam

[GreyRockIQ]

Hi @samuel-marsh
Thanks
Worked with restart and reload
Thanks
GreyRock